摘要
本研究以田黄李叶片总DNA为材料,分析了DNA模板浓度、循环次数、退火温度和引物浓度等因素对李ISSR-PCR效果的影响。根据试验结果建立李ISSR-PCR扩增的最佳反应体系。试验结果表明,当模板DNA量为40 ng,引物浓度为0.3或0.4μM,退火温度为48.8℃,循环数为35或40时可获得较好的扩增效果。
In the present study, effects of factors, including concentration of DNA template, cycle number, annea-ling temperature and concentration of primer, were investigated using total DNA from plum leaves. The optimal ISSR - PCR reaction condition for plum was determined. The results indicated that a good result can be obtained when 40 ng DNA and 0. 3 or 0.4 M primer was used, anneal at 48.8℃ and cycled for 35 or 40 times.
出处
《福建果树》
2012年第4期16-19,共4页
Fujian Fruits
基金
福建省农业科学院"双百"项目(sbmb1242)
农业部公益类行业科研专项(201003058-8)
福建省公益类科研院所专项(2011R1016-2)
福建省财政专项(CXTD2011-19)
