摘要
目的观察吉兰-巴雷综合征（GBS）脑脊液（CSF）中14—3—3蛋白与脑脊液蛋白在病程中的变化情况，探讨两者的相关性。方法采用酶联免疫吸附试验法（ELISA）检测43例GBS患者发病后3d内、第1，2，4，12周的脑脊液14—3—3蛋白含量，并同时用罗氏P-800全自动生化分析仪检测脑脊液蛋白含量。观察脑脊液14—3—3蛋白与脑脊液蛋白是否具有相关性。结果GBS组在发病3d内、第1，2，4，12周脑脊液14—3—3蛋白含量分别为（6．339±2．356）、（8．569±3．454）、（14．561±6．621）、（9．563±3．486）、（6．262±2．643）ng／ml，脑脊液蛋白分剐为（O．336±0．126）、（O．368±0．102）、（0．653±0．246）、（0．446±0．178）、（0．364±0．158）g／L。GBS组脑脊液14—3—3蛋白第1、2、4周高于对照组，差异有统计学意义（P〈0．05），GBS组脑脊液蛋白第2、4周高于对照组，差异有统计学意义（P〈0．05），脑脊液14—3—3蛋白含量与脑脊液蛋白含量存在正相关关系（P〈0．05）。结论GBS脑脊液中14—3—3蛋白含量的变化可引起脑脊液蛋白含量发生相应变化。
Objective To observe the variation of 14--3--3 protein and cerebrospinal fluid protein during the process of Guillain--Barre Syndrome and explore their correlation. Methods ELISA kit was used to measure the amount of 14--3--3 protein of the cerebrospinal fluid in 43 patients with Guillain-- Barre Syndrome at following time points： within the first 3 days, at weeks 1, 2, 4, and 12. Roche P-- 800 fully automatic biochemical analyzer was used to test protein of the cerebrospinal fluid at the same time points. The correlation between 14--3--3 protein and cerebrospinal fluid protein was analyzed. Re- suits The concentration of 14--3--3 protein in the eerebrospinal fluid were （6. 339±2. 356）, （ 8. 569± 3.454 ）,（14.561±6.621）,（9.563±3.486）,（6. 262±2. 643）ng/ml at weeks 1, 2, 4, and 12, respectively. Cerebrospinal fluid protein levels were （0. 336 ±0. 126）, （0. 368 ± 0. 102）, （0. 653 ±0. 246）, （0.446±0.178）,（0.364±0. 158）g/l at weeks 1, 2, 4, and 12, respectively. 14--3--3 protein increased at the 1st week,the 2nd week,the 4th week as compared to that of the control group （P〈 0.05）. Cerebrospinal fluid protein increased at the 2nd week and the 4th week as compared to that of the control group （P 〈 0.05）. An obviously positive correlation was noted between 14--3--3 protein and eerebro- spinal fluid protein. Conclusions In Guillain--Barre Syndrome, the amount of cerebrospinal fluid protein was fluctuating with 14-- 3-- 3 protein.
出处
《神经疾病与精神卫生》
2013年第1期57-59,共3页
Journal of Neuroscience and Mental Health
