摘要
目的:检测脑红蛋白(neuroglobin,Ngb)在人脑胶质瘤中的表达并探讨其临床意义。方法:2011年5月至2012年3月在中南大学湘雅医院及湘雅三医院病理科收集胶质瘤样本98例,应用免疫组织化学SP法检测脑红蛋白在不同组织学类型及WHO级别的胶质瘤中的表达情况及其与Ki-67表达的关系。结果:98例脑胶质瘤中,Ngb的总阳性率为88.78%,Ki-67均出现不同程度的阳性表达。1)按组织学类型分类,发现63例星形胶质细胞瘤中,Ngb表达阴性、弱阳性、中度阳性、强阳性的例数分别为2、20、26和15例;9例少突细胞胶质瘤均为阴性表达;15例少突星形细胞瘤中13例呈弱阳性表达,2例为中等阳性表达;11例室管膜肿瘤中,中等阳性表达有9例,强阳性表达为2例,经非参数检验发现,四种组织学类型中Ngb的阳性程度均存在差异,具有统计学意义(P<0.05)。2)按WHO分级分类,发现11例Ⅰ级胶质瘤中2例Ngb呈阴性表达,9例为弱阳性表达;39例Ⅱ级胶质瘤中Ngb表达阴性、弱阳性、中度阳性、强阳性的例数分别为5、13、16和5例;而在29例Ⅲ级中分别为4、11、11和3例;19例Ⅳ级胶质瘤中10例呈中等程度阳性表达,9例呈强阳性表达,经Kruskal-Wallis秩和检验发现,随胶质瘤WHO级别越高,Ngb的阳性程度越高,各WHO级别之间Ngb的阳性程度差异均具有统计学意义(P<0.05)。3)Ki-67与Ngb的相关分析发现,不同阳性程度的Ngb的表达与核增殖指数Ki-67之间存在正相关关系(rs=0.47,P<0.05)。结论:通过对不同组织学类型和不同WHO级别的胶质瘤中Ngb的表达及其与Ki-67指数关系的研究,表明Ngb在胶质瘤的诊断、治疗、评价恶性程度和预后中有重要意义,尤其在胶质瘤的治疗中有广阔的发展前景。
Objective： The present study aimed to detect the expression and clinical significance of neuroglobin （Ngb） in glio- mas. Methods： The immunohistochemical method was used to detect the expression of Ngb in gliomas of different histological types and WHO degrees as well as the correlation between Ngb and the Ki-67 positive index. Results： The total positive rate of Ngb in the gliomas was 88.76%. 1） According to the four different histological types of gliomas, among the 63 cases of astrocytoma, Ngb was ex- pressed as negative, weak positive, moderate positive, and strong positive in 2, 20, 26, and 15 cases, respectively. The nine cases of oli- godendrocytes glioma were all negative for Ngb expression. A total of 13 out of the 15 oligoastrocytoma cases showed a weak positive expression of Ngb, whereas the other two cases showed a moderate positive Ngb expression. Out of the 11 ependymoma cases, nine were moderate positive cases and two were strong positive cases. The expression levels of Ngb were significantly different （P〈0.05） among the four different histological types of gliomas. 2） According to the different WHO levels of gliomas, two gliomas of WHO I de- gree were negative for Ngb expression, whereas the other nine cases showed a weak positive expression of Ngb. Among the 39 gliomas of WHO II degree, Ngb was expressed as negative, weak positive, moderate positive, and strong positive in 5, 13, 16, and 5 cases, re- spectively, whereas among the 29 gliomas of WHO III degree, the number of cases were 4, 11, 11, and 3. Ten cases exhibited moderate positive Ngb expression, whereas nine cases showed strong positive expression for the WHO IV degree. Ngb expression levels between every two different WHO degrees were significantly different （P〈0.05）. A higher WHO degree generates a stronger Ngb expression. 3） Ngb has a significant correlation with Ki-67. Ngb is positively correlated with the Ki-67 positive index （rs=0.47, P〈0.05）. Conclusion： The expression level in gliomas of different histological types and WHO degrees and the significant correlation between the expression level of Ngb and Ki-67 positive index show that Ngb plays a role in the diagnosis, therapy, judgment of malignant degree, and progno- sis of gliomas. Ngb can be further developed to treat gliomas.
出处
《中国肿瘤临床》
CAS
CSCD
北大核心
2013年第6期328-331,340,共5页
Chinese Journal of Clinical Oncology
