摘要
目的探讨梅毒血清复发与神经梅毒之间的关系,同时从细胞免疫学角度对梅毒血清复发的发生机制进行研究。方法对20例符合梅毒血清复发诊断的患者行腰穿抽取脑脊液,进行常规、生化、血浆快速反应素试验(RPR)及梅毒螺旋体明胶凝集试验(TPPA)检测;采用双抗体夹心酶联免疫吸附试验(ABC-ELISA)分别检测血清复发组、RPR阴转组(20例)和正常人群组(20例)血清中γ-干扰素(IFN-γ)的含量,以及血清复发组脑脊液中IFN-γ的含量。结果在20例血清复发患者中,有5例神经梅毒(25.0%)。血清复发组患者血清IFN-γ水平明显低于正常对照组、RPR阴转组,差异均有统计学意义(P<0.01,P<0.01)。结论神经系统受累可能是梅毒血清复发的原因之一;梅毒血清复发患者的细胞免疫功能受到抑制。
Objective To explore the relationship between serum recurrence and neurosyphilis,and to investigate the pathogenesis of serum recurrence in a cellular immunity perspective.Methods Lumbar puncture was performed to collect cerebrospinal fluid（CSF） samples among 20 syphilitic patients with serum recurrence for routine and biochemical analysis,rapid plasma regain（RPR） assay and treponema pallidum particle agglutination（TPPA） test.Double antibody sandwich enzyme-linked immunosorbent assay（ELISA） was used to detect the content of interferon-γ（IFN-γ） in the cerebrospinal fluid and sera of patients,RPR negative group and normal controls.Results Five out of 20 patients were diagnosed as neurosyphilis（25.0%）.The sera IFN-γ level in the serum recurrence group was remarkably lower than in the RPR negative group（P0.01） and normal controls（P0.01）.Conclusion Nervous system involvement may be one of the causes of syphilis serological recurrence.The cellular immunity is suppressed in the syphilitic patients with serum recurrence.
出处
《中国艾滋病性病》
CAS
2013年第3期195-197,共3页
Chinese Journal of Aids & STD
关键词
梅毒
血清复发
Γ-干扰素
细胞免疫
Syphilis
Serum recurrence
Interferon-γ
Cellular immunity
