摘要
目的建立HPLC柱后光化学衍生法检测中药材中黄曲霉毒素G2、G1、B2、B1方法。方法样品经过70%甲醇提取、免疫亲和柱净化后,采用HPLC柱后光化学衍生-荧光检测器检测中药材黄曲霉毒素含量。对疑似成分进行液质确认。结果黄曲霉毒素G2和B2,G1和B1分别在0.75～22.5 pg和5～75pg线性关系良好,方法准确稳定。检测的34批次药材中,3批酸枣仁检出黄曲霉毒素,其中1批酸枣仁黄曲霉毒素B1超过5μg·kg-1。结论该方法简便、准确,适用于中药材黄曲霉毒素的检测。
Objective To establish HPLC methods associated with post column Photochemical Derivatization to determine aflatoxin G2,G1,B2,B1 in Chinese herbal.Method Aflatoxins were extracted by 70% methanol and purified by an immunoaffinity column.Then the samples were analysed by HPLC fluorescence detector with post column Photochemical derivatization.Confirm the suspected components with LC-MS.Results The method with the great linear concentration range of 0.75-22.5 pg for aflatoxins G2,B2,and 5-75 pg for aflatoxins G1,B1 respectively,was stable and accurate.In the result of 34 batches of Chinese herbs,Aflatoxins were detected in 3 batches of Semen Ziziphi Spinosae among which Aflatoxin B1 of one batch exceed 5 μg·kg-1.Conclusion The method is simple and accurate,which is suitable for the determination of aflatoxins in Chinese herbal.
出处
《现代中药研究与实践》
CAS
2013年第1期43-47,共5页
Research and Practice on Chinese Medicines
关键词
黄曲霉毒素
免疫亲和柱
光化学衍生
aflatoxins
immunoaffinity column
photochemical derivatization
