摘要
目的排查生化分析仪上对血清钙(Ca)检测产生负干扰的试剂交叉污染项目,并分析原因。方法在HITACHI 7600全自动生化分析仪上,将18项常规生化项目与Ca配对组合检测,筛选出干扰明显的施污染项目,再将施污染试剂直接加入血清中,将试剂-血清混合物置生化仪上分析Ca浓度,根据Ca浓度是否明显偏离确定该试剂是否存在干扰。对各试剂成分进行调研,分析造成Ca负干扰的因素。结果 TBIL(钒酸盐法)、DBIL(钒酸盐法)、ALT、AST、HBD和GLU对Ca存在明显试剂负干扰,原因可能是EDTA、EGTA、柠檬酸盐等试剂添加剂致Ca失活。结论 Ca的负干扰因素多而抗干扰能力较低,日常工作中应注意重点防范。
Objective To investigate reagent contamination factors of serum calcium （Ca） detection with negative interference on biochemical analyzer, and to analyze the reasons. Methods On HITACHI 7600 automatie biochemical analyzer, 18 routine biochemical projects and Ca paired combined detection were performed. Significant interfere with the implementation of pollution projects were screened, and then the pollution reagents were directly added to the serum. The reagent-serum mixture was analyzed for Ca concentration by bioehemical analyzer. The apparent deviation from the reagent interference was determined based on Ca concentration. The reagent contamination fac- tors with negative interference were analyzed. Results TBIL （the vanadate Act）, DBIL （vanadate law）, ALT, AST, HBD and GLU exerted obvious negative interference on Ca. The reason might be that to the application of EDTA, EG- TA, eitrate reagent induced inactivation of Ca. Conclusion Ca has lots of negative interference factors and low anti-interference ability, which should be paid attention to in daily work.
出处
《海南医学》
CAS
2013年第8期1170-1171,共2页
Hainan Medical Journal
关键词
钙
负干扰
交叉污染
螯合剂
Calcium
Negative interference
Cross-contamination
Chelating agents
