摘要
目的探讨Hedgehog信号通路、Wnt信号通路关键组成成员Glil和β—catenin在大肠癌组织及肠癌细胞系中的表达协同情况，以及两种蛋白之间的相互作用关系。方法采用荧光定量反转录聚合酶链反应（RT—PCR）结合Western blot方法检测Gli1、β—catenin在4例大肠癌组织及肠癌HCT116、HT-29、DLD1、SW620细胞系中表达情况，应用免疫共沉淀技术检测二者之间是否存在相互作用。结果与癌旁正常组织相比，Gli1和β—eatenin在大肠癌组织中均高表达[平均增长倍数分别为42．69和72．11（P〈0．05）]。与HCT116相比，Gli1与β-catenin在HT-29、DLD1及SW6203株大肠癌病变程度高的细胞中表达均升高[士曾长倍数分别为52．54、17．23、5．54及5．30、6．34、2．78（均P〈0．05）]，二者的表达具有协同性。免疫共沉淀结果显示Glil与β—catenin两蛋白之间存在相互作用。结论Glil和β-eatenin在大肠癌中协同表达，蛋白之间存在相互作用，提示Glil、β—catenin共同参与肠癌发生、发展调控，可能是Hedgehog、Wnt信号通路交互作用的一条途径，对大肠癌的预防治疗可能有提示作用。
Objective To investigate the expression of Glil and β-catenin, which are central molecules of Hedgehog and Wnt signaling pathway, in colon cancer tissues and cell lines, and evaluate their relationship. Methods RT-PCR and Western blot were used to determine the expression of Glil and β-catenin in colon cancer tissues and cells. Interaction between endogenous Glil and endogenous β-catenin was examined using immunoprecipitation method. Results Compared to corresponding normal tissues, the expression of Glil and β-catenin raised raising with one accord, the average growth rates were 42.69, 72.11 respectively （P 〈 0.05）. In colon cancer cell lines, the expression levels of Glil and β-eatenin increased, which were 52.54, 17.23, 5.54 and 5.30, 6.34, 2.78 （all P 〈 0.05）. Both of them were enhanced in three Wnt aberrant active cells visibly. The immunoprecipitation result indicated that there was an interaction between Glil and β-catenin in colon cancer cells. Conclusion The expression of Glil and β-catenin rise congruously in both colon cancer ceils and tissues, and there is an interaction between them. This interaction between two key components of Hedgehog and Wnt signaling may suggest the possible crosstalk manner in these pathways.
出处
《肿瘤研究与临床》
CAS
2013年第3期175-177,共3页
Cancer Research and Clinic
