摘要
质粒 pGA46 4带有从谷氨酸棒杆菌染色体上分离到的有启动功能的片段 ,用PCR技术从 pGA46 4中扩增了该片段的关键区域 :启动子PGL.将该启动子经EcoRⅠ BamHⅠ双酶切后 ,与大肠杆菌质粒 pJL0 1的EcoRⅠ BamHⅠ大片段连接 ,再接入XylEgene和棒状杆菌质粒pXZ 10 142 ,构建成棒状杆菌 大肠杆菌穿梭表达载体 pJL2 3.用邻苯二酚双加氧酶基因检测表明 ,该表达载体可在棒状杆菌中高效地表达外源基因 .
Plasmid pGA46 4 contains a promoter function fragment isolated from the chromosome of Corynebacterium glutamicum . The essential part of this fragment promoter P GL is amplified from pGA46 4 by PCR. In order to construct a shuttle expression plasmid pJL23, this promoter was ligated with Escherichia coli plasmid pJL01, the Xyl E gene and the corynebacteria plasmid pXZ10142. According to the Catechol 2,3 dioxygenase expression results, the expression vector functions well in the expression of foreign genes in corynebacteria.
出处
《复旦学报（自然科学版）》
CAS
CSCD
北大核心
2000年第3期292-296,共5页
Journal of Fudan University：Natural Science
