摘要
1目的 探讨缩胆囊素 (CCK)和缩宫素 (OT)神经元在下丘脑的共存现象。 2方法 采用相邻切片免疫组化方法 ,检测 CCK和 OT样免疫活性物质 (CCK- IR,OT- IR)的分布。 3结果 CCK- IR广泛分布在下丘脑的视上核、室周核、视交叉上核、腹内侧核和弓状核等区域 ,由中等大小卵圆形细胞构成 ,室旁核未检测到 CCK- IR;OT- IR仅局限于室旁核和视上核 ,由大细胞构成。 4结论 CCK和 OT存在于下丘脑不同类型的细胞中 ,且两种细胞仅共存于视上核。
Objective To study the distribution of CCK immunoreactive(CCK IR) and OT immunoreactive(OT IR) cells in the hypothalamus of rats.\ Methods\ Using the mirror sectioning immunohistochemical technique, the coexistence of CCK IR and OT IR cells in hypothalamus was examined with ABC immunostaining method on adjacent mirror thin sections.\ Results\ CCK IR cells distributed mainly in supraoptic nucleus(SON), suprachiasmatic nucleus(SCN), periventricular hypothalamic nucleus (PVN), arcuate hypothalamic nucleus(ARCN)and ventromedial hypothalamic nucleus(VMH); OT IR cells distributed only in SON and PVN.\ Conclusion\ This study reveals that CCK IR cells and OT IR cells co exist in SON, but they do not present in the same cells,for CCK IR cells are parvocellular cells,while OT IR cells are magnocellular cells. [
出处
《青岛大学医学院学报》
CAS
2000年第4期261-262,共2页
Acta Academiae Medicinae Qingdao Universitatis
基金
山东省卫生厅科研基金!资助项目 (鲁卫科计字[1994] 2 0号 )
关键词
下丘脑
缩胆囊素
缩宫素
免疫组织化学
大鼠
hypothalamus
cholecystokinin
oxytocin
immunohistochemistry
rats
