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利用流式细胞仪检测定性牛精液的分离效果 被引量：1

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摘要 利用德国生产的 PARTEC流式细胞仪，对使用 Percoll不连续密度梯度离心技术分离出的荷斯坦牛定性精液进行了定性检测，结果表明原精液中， X－与 Y－精子的比例基本为 1∶ 1，富含 X－精子的精液中 X－与 Y－精子的比例为 1.26∶ 1，富含 Y－精子的精液中 X－与 Y－精子的比例为 0.73∶ 1。
作者 李慧斌 韩建林 徐林平 曹世祯
机构地区 兰州市畜牧兽医研究所 甘肃农业大学
出处 《中国奶牛》 2000年第6期16-17,共2页 China Dairy Cattle
关键词 流式细胞仪 检测 定性 牛精液
分类号 S823.34 [农业科学—畜牧学]
  • 相关文献

参考文献10

  • 1曹世祯等.利用Percoll密度梯度离心技术分离奶牛的X—和Y—精子[J].中国动物遗传育种研究进展,1997,.
  • 2,P317-319.中国农业科技出版社.
  • 3曹世祯,韩建林,陈亮,颜宗舜.用Percoll不连续密度梯度分离奶牛精液对控制性别比率的研究[J].中国奶牛,2000(1):19-20. 被引量：13
  • 4Dilla, van M. A.,B. L. Gledhill, S. Lake, P. Deam, J. Gray,V. Kachel. B. Barlogie and W. Gohde, 1977, Measurement of mammalian sperm deoxyribonucleic acid by flow cytome-try. J. of Histochemistry and Cytochemistry, 25 (7):763～773.
  • 5Gledhill, L. B., S. Lake, L. L. Steinmetz, J. Gray,J. R. Crawford, P. Dean, and M. van Dilla, 1976. Flow mi-crofluorometric analysis of sperm DNA content: effect of cell shape on the fluorescence distribution. J. Cell Physiol-ogy, 87:367～376.
  • 6Johnson, L. A. and R. N. Clarke, 1988, Flow sorting ofXand Ychromosome-bearing mammalian sperm: activa-tion and pronuclear development of sorted bull, boar, and ram sperm microinjected into hamster oocytes. Gamete Re-search, 21:335～354.
  • 7Johnson, L. A., 1988. Flow cytometric determination of sperm sex ratio in semen purportedly enriched for X-orY-bearing sperm. Theriogenology, 29 (1): 265.
  • 8Johnsom, L. a.,J. P. Flook and H. W. Hawk,1989. Sex preselection in rabbits: live births from X and Y sperm separated by DNA and cell sorting. Biology of Reproduc-tion, 41: 199～203.
  • 9Morrell, J. M.,K. D. Keeler,D. E. Noakes,N. M. Mackenzie, D. W. Dresser, 1988, Sexing of sperm by flow cytometry. The Veterinary Record, 122:322～324.
  • 10Pinkel, D.,D. L. Garner, B. L. Gledhill, S. Lake,D. Stephenson and L. A. Johnson, 1985. Flow cytometric determination of the proportions of X-and Y-chromosome-bearing sperm in samples of purportedly separat-ed bull sperm. J. of Animal Science, 60(5): 1303～1307.

二级参考文献3

共引文献12

同被引文献10

  • 1Sinclair A H et. al. A gene from the human sex-determining region encides a protein with homology to a conservered DNA-binding motid [J]. Nature, 1990,346:240-244.
  • 2Johnson R C. Correction of semen variables and regnanvy rates for donor insemination: a 15-year retrospective[J]. Fertil Steril , 19,94,61:355-359.
  • 3Johnson L A, Clarke R N. Flow sorting of X and Y chromosome -bearing mammalian sperm: activation and pronuclear development of sorted bull, boar, and ram sperm microinjected into hamster oocytes[J]. Gamete Research, 1988,21: 335-354.
  • 4Johnson L A, Flow cytometric determination of sperm sex ratio in semen purportedly enriched for X-or Ybearing sperm[J]. Theriogenology,1988, 29(1):265.
  • 5Johnson I. A, Flook J P, Hawk H W, Sex preselection in rabbits: live births from X and Y sperm separaled by DNA and cell sorling[J].Biology of Reproduction, 1989,41: 199-203.
  • 6Hamano, Ki. Sex preselection by flow cytometric sorting X- and Y-chromosome bearing sperm cattle [J].Journal of the Faculty of Agriculture, 1999,35(2) :99-104.
  • 7Wachtel. Measurement of mammalian sperm by flow cytometry[J]. Theriogenology, 1975,21:16-27.
  • 8Kamentsky L A, Mclamed R R. Spectrophotometric cell sorter[J].Science, 1976,156:1364-1365.
  • 9Sukhato P. Sex determination of bovine embryos using a simplified cytogenetic technique[J]. Journal of the Veterinary Medical Association, 1997,48 (2) : 35-43.
  • 10贾福德,罗承浩,马俊洪.应用生殖免疫方法制作性别化冷冻精液对奶牛性别控制的研究[J].动物科学与动物医学,2001,18(2):14-17. 被引量：6

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中国奶牛
2000年 第6期

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