摘要
目的:研究灯盏乙素苷元对帕金森病(PD)模型大鼠多巴胺能神经元的保护作用。方法:通过给予大鼠脑立体定位注射脂多糖(LPS)复制PD模型。实验分为空白对照(等容生理盐水)、假手术(等容生理盐水)、模型(等容生理盐水)、美多芭(0.034mg/kg)与灯盏乙素苷元高、中、低剂量(800、400、200mg/kg)组。灌胃给药,每天1次,连续3周。背部皮下注射阿朴吗啡诱导进行行为学观察;测定丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽(GSH)水平。结果:与模型组比较,灯盏乙素苷元高、中、低剂量组大鼠旋转速度显著减慢(P<0.05);SOD活性显著增强,GSH含量显著增加,MDA含量显著减少(P<0.01或P<0.05)。结论:灯盏乙素苷元能改善PD模型大鼠的旋转行为,同时可以提高多巴胺能神经元的抗氧化能力,从而对PD大鼠的中枢神经系统发挥保护作用。
OBJECTIVE： To explore the protective effect of scutellarein on dopaminerigic neurons of parkinson＇s disease （PD） model rats. METHODS： PD rat models were induced by stereotaxic injection of LPS. Model rats were divided into 7 groups, i.e. blank control group （constant volume of normal saline）, sham operation group （constant volume of normal saline）, model group （constant volume of normal saline）, Madopar group （0.034 mg/kg） and scutellarein high-dose, medium-dose and low-dose groups （800, 400, 200 mg/kg）. They were given medicine intragastrically once a day for consecutive 3 weeks. Apomorphine （apo） was injected subcutaneously on the rat back to observe praxiology of it. The levels of MDA, SOD and GSH were determined. RESULTS ： Compared with model group, rotational speed of rats were decreased significantly in scutellarein high-dose, medium-dose and low-dose groups（P〈0.05） ; GSH and SOD levels were increased significantly, while MDA level was decreased significantly（P〈 0.01 or P〈0.05）. CONCLUSION： Scutellarein can improve rotation behavior of PD model rats and the ability of anti-oxidation of dopaminergic neurons and protect the central nervous system of PD rats.
出处
《中国药房》
CAS
CSCD
2013年第15期1360-1362,共3页
China Pharmacy
