摘要
背景:益气化瘀是中医药对脊髓损伤的主要治则,但疗效不确切。那么,益气活血类中医药治法能否结合现代科技共同治疗脊髓损伤从而提高疗效呢?目的:观察益气活血类汤剂促进嗅鞘细胞增殖及分泌神经生长因子、脑源性神经营养因子的作用。方法:组织块培养法体外培养新生大鼠嗅鞘细胞;采用MTT法检测补阳还五汤和痿症方含药血清对嗅鞘细胞增殖的影响;采用Western-Blot法测定培养上清中神经生长因子、脑源性神经营养因子水平。结果与结论:MTT结果显示补阳还五汤药物血清及痿证方药物血清均能促进嗅鞘细胞增殖,且两者促增殖作用无差异;Western-Blot结果显示补阳还五汤药物血清及痿证方药物血清均能促进嗅鞘细胞分泌神经生长因子、脑源性神经营养因子,两组间细胞因子分泌水平也无明显差异。
BACKGROUND： Supplementing qi and removing blood stasis is the main therapeutic principle of traditional Chinese medicine for the treatment of spinal cord injury, but the efficacy is not plausible. But, whether the application of serum-containing drugs responsible for supplementing qi and activating blood circulation under the guidance of modern technology can treat spinal cord injury and improve the efficacy？ OBJECTIVE： To study the effect of serum-containing drugs responsible for supplementing qi and activating blood circulation on the proliferation of olfactory ensheathing cells and the secretion of nerve growth factor and brain-derived neurotrophic factor. METHODS： The olfactory ensheathing cells were in vitro cultured with tissue block culture method. Theeffect of Buyanghuanwu decoction and Weizheng decoction on the proliferation of olfactory ensheathing cells was detected with MTT assay. The expression levels of nerve growth factor and brain-derived neurotrophic factor in the culture supernatant were detected by western blot assay. RESULTS AND CONCLUSION： MTT results showed that both Buyanghuanwu decoction and Weizheng decoction could promote the proliferation of olfactory ensheathing cells, and there was no significant difference in proliferation-promoting effect. Western blot assay results showed that both Buyanghuanwu decoction and Weizheng decoction could promote the expression of nerve growth factor and brain-derived neurotrophic factor in olfactory ensheathing cells, and there was no significant difference in secretion level between two groups.
出处
《中国组织工程研究》
CAS
CSCD
2013年第6期1094-1100,共7页
Chinese Journal of Tissue Engineering Research
关键词
干细胞
干细胞与中医药
补阳还五汤
痿证方
益气活血
含药血清
嗅鞘细胞
增殖
神经生长因子
脑源性神经营养因子
促增殖
组织修复
干细胞图片文章
stem cells
stem cells and traditional Chinese medicine
culture and differentiation
Buyanghuanwudecoction
Weizheng decoction
supplementing qi and activating blood circulation
drug-containing serum
olfactory ensheathing cells
proliferation
nerve growth factor
brain-derived neurotrophic factor
proliferationpromotion
tissue repair
photographs-containing paper of stem cells
