摘要
以分子生物学和遗传学为基础,探索建立了山羊球鞘毛尾线虫特异性PCR检测方法,并进行了敏感性、特异性及虫体鉴定应用试验。结果表明,该方法可扩增出毛尾线虫长度为1 500bp左右的目的片段;特异性PCR可将山羊球鞘毛尾线虫与山羊粗纹食道口线虫、哥伦比亚食道口线虫、捻转血矛线虫、奥拉奇细颈线虫、美丽筒线虫有效区分;敏感性试验结果表明,该PCR可检测毛尾线虫的最低DNA模板质量浓度为0.82ng/μL。用该特异PCR检测方法对陕西4个地方的毛尾线虫随机抽取10条进行分析,检测结果与传统的形态学鉴定结果相符,但比传统方法更加简捷、快速和准确。
A specific PCR assay for detecting goat Trichuris globulosa was established. The specificity, sensitivity and applicability were studied. The results showed that the PCR method could amplify about 1 500 bp fragment with the lowest detection limit of 0.82 ng/μL DNA of T. globulosa. The PCR was speci- fic to effectively distinguish T. globulosa from Oesophagostornurn asperurn, Oesophagostornum columbia- nurn, Haemonchus contortus, Nernatodirus oiratianus and Gongylonerna pulchrurn. The application for de- tecting 10 goat T. globulosa isolates, randomly collected from four regions in Shaanxi Province, showed that consistent results with that of morphological identification, but the PCR was faster, more convenient and efficient.
出处
《中国兽医科学》
CAS
CSCD
北大核心
2013年第3期294-298,共5页
Chinese Veterinary Science
基金
神木县羊寄生虫病防治技术研究(西北农林科技大学K332021210)
西北农林科技大学基本科研业务费重点项目(ZD2012010)
