摘要
目的：探讨野生型ｐ５３(ｗｔ ｐ５３)基因对人卵巢癌细胞株的生长抑制作用。方法：利用脂质体介导，将含有人全长ｗｔ－ｐ５３基因ｃＤＮＡ的真核表达载体质粒ｐＣＥＰ４／ｐ５３和空载体质粒ｐＣＥＰ４分别转染卵巢癌ＳＫＯＶ３细胞株，分别命名为ＳＫＯＶ３－ｐＣＥＰ４／ｐ５３细胞(C组)、ＳＫＯＶ３－ｐＣＥＰ４细胞(B组)，另设ＳＫＯＶ３细胞为正常对照组（Ａ组）。观察细胞体外生长情况和凋亡变化。结果：外源性ｐ５３基因在Ｃ组细胞中获表达，细胞生长曲线显示ｐ５３的导入使ＳＫＯＶ３细胞生长受到明显抑制，Ｃ组平均细胞集落数（１８．６±１．８个）少于Ａ组（２４．３±２．２个）和Ｂ组（２２．７±２．６），差异有显著性（Ｐ（005）。ＭＴＴ法检测C组细胞活力明显低于Ａ、Ｂ组。Ｃ组细胞Ｇ０～Ｇ１期百分比（５７．７９％）及凋亡细胞百分比（13．９1％）均高于Ｂ组（４６．０２％、２．０８％）和Ａ组（４３．６２％、０）。结论：ｗｔ－ｐ５３基因可介导ＳＫＯＶ３细胞Ｇ１期停滞和细胞凋亡，抑制细胞体外生长。
Objective: To study the inhibit ory effects of wild type p53 gene on hum an ovarian cancer cellsMethods: Recombinant eukaryotic expression vector pCEP4/p53 containing full-length strain s wild type p53 cDNA and vector pCEP4 co ntaining hygromycin resistance only were introduced by lipofectamine transfectio n regent into SKOV3 cell strains which d o not express endogenous p53Resul ts:wt p53 was successfully transferred into SKOV3 cells and obtained high expression. The introduction of exogenous wt p53 into tumor cells resulted in mar ked growth inhibition.MTT assay showed t hat the cell viability decreased significantlyThe percentage of phase G0～G1(57 79%) and that of apoptosis(1391%) of SKO V3 cells transfected with pCEP4/p53 were much higher than that of normal SKOV3 c ells(4362%,0)and SKOV3 cells transfected with pCEP4(4602%,208%)Conclusions: wt p53 induces phase G1 arrest and apoptosis of SKOV3 cell strains, which may result in the inhibition of cell growth
出处
《现代妇产科进展》
CSCD
2000年第4期246-249,共4页
Progress in Obstetrics and Gynecology
关键词
卵巢肿瘤
P53基因
转染
细胞凋亡
Human ovarian neoplasms,Gene,p53
Transfection
Apoptosis
