摘要
为研究成人骨髓基质细胞的体外培养及其成骨特性 ,将来自需取髂骨的 9例患者的骨髓标本进行体外培养 ,传代细胞进行含 10 - 8mmo1· L- 1 地塞米松的条件培养和不含地塞米松的非条件培养。倒置显微镜每日观察细胞生长情况 ,计数传代细胞中骨样细胞转化率 ,检测碱性磷酸酶含量。 9例标本骨髓基质细胞培养均获得成功 ,条件培养后有较高的成骨细胞转化率及碱性磷酸酶活性。结果表明 ,成人骨髓基质细胞条件培养后 。
WT6BZ]In order to study the in vitro culture of adult bone marrow stroma cells and its osteogenic characteristics, nine bone marrow specimens from the ilia of the patients receiving iliac grafting were cultured in vitro, and the subcultures were cultured with conditions containing 10 8 mmol. L 1 dexamesthasone (DXM) and with no conditions. The every day growth of the cells was observed with inverted microscope, the transmission rates of the ossiform cells in the subcultured cells were counted, and the contents of AKP were measured. The bone marrow stroma cells were successfully cultured in all the nine specimens. A higher transmission rate of osteoblasts and a higher AKP activity were obtained in the culture with conditions. The results showed that the adult bone marrow stroma cells cultured with conditions may offer a source of the osteogenous seed cells for the clinical requirement.
出处
《中医正骨》
2000年第5期5-6,共2页
The Journal of Traditional Chinese Orthopedics and Traumatology
关键词
骨
细胞学
骨髓基质细胞培养
实验
bone/cytology bone marrow stroma cell culture/methology experimental study man (Original article on page 5)
