摘要
目的探讨骨髓来源间充质干细胞(MSCs from bone marrow,BMSCs)与脂肪来源间充质干细胞(Adipose tissue-derived MSCs,ADSCs)体内移植后的抗原性差异。方法体外培养WKY大鼠BMSCs、ADSCs,经成骨诱导后植入wistar大鼠桡骨缺损区,分别于植入后1、2、4、8周进行缺损区HE染色,于1、2、4、8周免疫组化检测缺损区IL-2、TGF-β、TNF-α、CD4、CD8,采用ELISA法检测脾细胞培养液上清IL-2、TGF-β、TNF-α的含量。结果 BMSCs、ADSCs移植术后1～2周有少量淋巴细胞、白细胞浸润,IL-2、TGF-β、TNF-α、CD4、CD8蛋白少量表达;二者比较差异无显著性。结论 BMSCs、ADSCs体内移植后所致的抗原性无显著性差异。
Objective to investigate the antigenic difference of allogeneic bone marrow-derived mesenchymal stem cells （BMSCs） and adipose tissue-derived mesenchymal cells （ADSCs） after their transplantation. Method BMSCs, ADSCs from WKY rats were cultured in vitro and were transplanted into the radial osseous defects in Wistar rats after osteogenic induction; 1,2,4、8 weeks after implantation, tissues from the defective areas was taken for HE staining, /L-2, TGF- β, TNF- a, CD4, CD8 were detected using immunohistochemical staining and quantity of IL-2, TGF- β, TNF- a was determined in supematant of spleen cell culture using ELISA. Results 1-2 weeks after transplantation of BMSCs, ADSC, mild lymphocyte, leukocyte infiltration can be observed in the defective areas, a small amount of IL-2, TGF- β, TNF- a, CD4, CD8 can be detected; there was no significant difference in the 2 groups. Conclusion No significant difference can be found in BMSCs, ADSCs after transplantation.
出处
《中国临床解剖学杂志》
CSCD
北大核心
2013年第2期174-179,共6页
Chinese Journal of Clinical Anatomy
基金
辽宁省教育厅科学技术创新团队课题(LT2010061)
国家自然科学基金(31170930)
关键词
同种异体
间充质干细胞
抗原性
骨缺损
Allogeneic
Mesenchymal stem cells
Antigenicity
Bone defect
