期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

“Golden Gate”克隆法构建靶向载体 被引量：4

Assembly of Targeting Vector by Golden Gate Cloning
原文传递
导出
摘要 在同一反应体系内,利用IIS型限制性核酸内切酶和DNA连接酶进行"Golden Gate"克隆方法,将多个目的基因片段按照设定的顺序连接,实现多基因片段一步"无缝"连接。为编辑小鼠β酪蛋白基因位点,利用"Golden Gate"克隆法,构建了小鼠β酪蛋白基因位点同源重组靶向载体。 The ＆lsquo;Golden Gate＆rsquo; cloning is based on the ability of type IIS restriction enzymes and DNA ligase enzymes to assemble multiple DNA fragments in a defined linear order in one tube and one step. The method is efficient and yields recombinant vectors that do not contain unwanted sequences in the final construct after just a short time restriction-ligation. To edit mouse ＆beta; casein gene, recombination targeting vectors of the mouse ＆beta; casein gene targeting locus were constructed by the ＆lsquo;Golden Gate＆rsquo; cloning.
作者 梁龙 杨华 杨永林 刘守仁 皮文辉
机构地区 石河子大学动物科技学院 新疆农垦科学院畜牧兽医研究所 新疆兵团绵羊繁育生物技术重点实验室
出处 《中国生物工程杂志》 CAS CSCD 北大核心 2013年第3期111-116,共6页 China Biotechnology
基金 兵团博士资金(2009JC05) 国家自然科学基金(31060159) 国家留学基金委"西部人才培养特别项目"([2009]5007)资助项目
关键词 DNA重组 &ldquo GOLDEN Gate&rdquo 克隆法 小鼠&beta 酪蛋白基因 DNA recombination ＆lsquo Golden Gate＆rsquo cloning Mouse ＆beta casein gene
分类号 Q78 [生物学—分子生物学]
  • 相关文献

参考文献10

  • 1Marsischky G, LaBaer J. Many paths to many clones: a comparative look at high-throughput cloning methods. Genome Research, 2004, 14:2020 - 2028.
  • 2Engler C, Kandzia R, Marillonnet S. A one pot, one step, precision cloning method with high throughput capability. PLoS ONE, 2008, 3 ( 11 ) : e3647.
  • 3Engler C, Gruetzner R, Kandzia R, et al. Golden gate shuffling: a one-pot DNA shuffling method based on type IIS restriction enzymes. PLoS ONE, 2009, 4(5) : e5553.
  • 4Vlorbitzer R, Elsaesser J, Hausner J, et al. Assembly of custom TALE-type DNA binding domains by modular cloning. Nucleic Acids Res, 2011, 39( 13 ) : 5790-5799.
  • 5Szybalski W, Kim S C, Hasan N, et al. Class-llS restriction enzymes--a review. Gene, 1991, 100: 13-26.
  • 6Weber E, Gruetzner R, Weruer S, et al. Assembly of designer TAL effectors by golden gate. PLoS One, 2011 , 6(5) : e19722.
  • 7Cermak T, Doyle E L, Christian M, et al. Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting. Nucleic Acids Res, 2011, 39 (12) : e82.
  • 8Sanjana N E, Cong L, Zhou Y, et al. A transcription activator-like effeetor toolbox for genome engineering. Nat Protoo, 2012, 7 (1) : 171-192.
  • 9G off S P, Berg P. Construction of hybrid viruses containing SV40 and lambda phage DNA segments and their propagation in cultured monkey cells. Cell, 1976, 9: 695.
  • 10Weber E, Engler C, Gruetzner R, et al. A modular cloning system for standardized assembly of muhigene constructs. PLoS One, 2011,6(2): e16765.

同被引文献27

  • 1杨惠杰,何花榕,杨聚宝,王侯聪.两种抗生素对水稻种胚愈伤组织诱导和分化的影响[J].福建省农科院学报,1994,9(4):5-10. 被引量：3
  • 2Gordon K, Lee E, Vitale JA, et al. Production of human tissue plasminogen activator in transgenic mouse milk [ J]. Nature Bio- technol. 1987, 5 : 1183 - 1187.
  • 3Park YW, Juarez M, Ramos M, et al. Physico-chemical charac- teristics of goat and sheep milk [J]. Small Ruminant Res. 2007, 68(1) :88 -113.
  • 4Richardson BC, Creamer LK. Comparative micelle structure. 3.The isolation and chemical characterization of caprine beta 1 -case- in and beta 2-casein [ J]. Biohem Biophys Acta. 1974, 365 (1) :133 -138.
  • 5Onoda M, Inano H. Distribution of casein-like proteins in various organs of rat [ J]. J Histochem Cytochem. 1997, 45 (5):663 - 674.
  • 6Grusby MJ, Mitchell SC, Nabavi N, et al. Casein expression in cytotoxic T lymphocytes [J]. Proc Natl Acad Sci U S A. 1990, 87(17) :6897 -6901.
  • 7Kumar S, Clarke AR, Hooper ML, et al. Milk composition and lactation of 13-casein-deficient mice [J]. Proc Natl Acad Sci U S A. 1994, 91(13) :6138 -6142.
  • 8Kolb AF, Ansell R, McWhir J, et al. Insertion of a foreign gene into the β-casein locus by Cre-mediated site-specific recombina- tion [J]. Gene. 1999, 227(1) :21 -31.
  • 9多曙光,吴应积,罗奋华,等.用体外培养的牛乳腺上皮细胞检测乳腺生物反应器表达载体的方法[C].中国生物工程学会第四次会员代表大会暨学术讨论会论文摘要集.2005年.
  • 10Joung JK, Sander JD. TALENs: a widely applicable technology for targeted genome editing [ J]. Nat Rev Mol Cell Biol. 2013, 14:49 - 55.

引证文献4

二级引证文献1

中国生物工程杂志
2013年 第3期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部