摘要
在"异养-胁迫"分段培养模式下分析原始小球藻(Chlorella protothecoides)的生物量及油脂含量。结果表明,在500ml摇瓶中,分段培养生物量达5.32g/L,略低于一步异养培养5.45 g/L,油脂含量达34.81%,是一步异养培养的2.26倍,藻渣中微藻多糖含量由9.57%提高到18.06%。在3L发酵罐中,一步异养培养模式下生物量为14.1 g/L,油脂含量为17.16%,微藻多糖含量为10.16%;而"异养-胁迫"分段培养模式下生物量13.20g/L,油脂含量40.15%,微藻多糖含量24.74%。本研究显示"异养-胁迫"分段培养模式是一种快速获得大量高油脂含量藻细胞的有效方法,可为规模化生产微藻生物柴油和进一步利用微藻奠定了技术基础。
A novel cultivation strategy, ＂heterotrophy - stress＂ two-step cultivation was developed to solve the problem of asynchronism between biomass and lipid production in Chlorella protothecoides research. The C. protothecoides was first cultivated in optimized heterotrophic medium to achieve high cell density with low lipid content. Then, the algal cells were washed, condensed and transferred to stress medium without nitrogen source in order to accumulate high lipid content. With this strategy, the C. protothecoides biomass in 500ml flask achieved 5.32 g/L dry-weight which was close to the level of traditional heterotrophic cultivation mode, but the lipid content increased from 15.40% to 34.81%, and the algal polysaccharide content of the residue after lipid extraction increased from 9.57% to 18.06%. Furthermore, a 3L fermenter experiment showed a consistent pattern. In nitrogen rich medium, the biomass reached 14.1 g/L dry-weight, lipid content reached 17.16%, and the algal polysaccharide content was 10.16%, while in ＂ heterotrophy - stress＂ two-step cultivation, the biomass attained 13.2 g/L dry-weight, the lipid attained 40.15%, and the algal polysaccharide content was 24.74%. Therefore, this study indicates that the proposed strategy may provide an effective approach for microalgal biomass production with high lipid content.
出处
《中国生物工程杂志》
CAS
CSCD
北大核心
2013年第3期99-104,共6页
China Biotechnology
基金
国家"863"计划(2011AA02A204)
国家自然科学基金(31070089
J1103514)资助项目
关键词
原始小球藻
生物量
油脂含量
分段培养
Chlorella protothecoides
Biomass
Lipid content
Two step cultivation
