摘要
摘要目的：比较两种不同血小板计数方法的准确性。方法：收集PLT〈100×10^9／L，在LH750机上检测，PLT计数无异常提示，50例标本（A组）。收集PLT〈100×10^9／L，在LH750机上检测，PIT计数异常提示，50例标本（B组）。排除血小板聚集对血小板计数影响，分别进行相差显微镜镜检法、Sysmex XE-5000PLT-I和PLT-O血小板计数比较。结果：A组数据进行比较，LH750血细胞计数仪PLT-I原理检测PLT，Sysmex XE-5000PLT-I原理检测PLT、PLT-O原理检测PLT，以及手工计数PLT，两两比较P〉0．05，差异无统计学意义。B组数据进行比较，LH750血细胞计数仪PLT-I原理检测PLT，Sysme xXE-5000PIJ-I原理检测PLT、P〉0．05差异无统计学意义。LH750血细胞计数仪PLT-I原理检测PLT，Sysmex XE-5000PLT-I原理检测PLT与Sysmex XE-5000PLT-O原理检测PLT比较，P〈0．05差异无统计学意义，Sysmex XE-5000PLT-0原理检测PLT与手工计数比较P〉0．05，差异无统计学意义。结论：对低异常血小板，尤其存在大血小板标本，为了避免电阻抗法血小板计数假性减少，可进行较为方便的PLT．O法计数加以纠正，必要时进行较为繁琐的镜检法计数，为临床提供更为准确的血小板数据。
Objective：To compare the accuracy between the two platelet count methods. Methods：PLT 〈 100×10^9/ L specimens selected and detected with no abnormal indications of PLT counts on LH-750 were assigned into group A, while PLT 〈 100 ×10^9/L specimens detected with abnormal indications of PLT counts on LH-750 were into group B （n = 50, each）. The PLT-I and PLT-O platelet counts were detected respectively by phase contrast microscope and Sysmex XE-5000, excluding the effects of platelet aggregation on platelet count. Results：In group A, PLT specimens were respectively detected by LH750 hematology analyzer PLT-I principle, Sysmex XE-5000 PLT-I principle, PLT-O principle and manual counting, with no significant difference （P 〉 0. 05 ）. While in group B, PLT specimens were respectively detected by LH750 hematology analyzer PLT-I principle and Sysmex XE-5000 PLT-I principle, with no significant difference （ P 〉 0. 05 ）. There was no difference among LH750 hematology analyzer PLT-I principle, Sysmex XE-5000 PLT-I principle and Sysmex XE- 5000 PLT-O principle （ P 〈 0.05 ） , nor between Sysmex XE-5000 PLT-O principle and manual counting （ P 〉 0. 05）. Conclusion ： For platelets with low abnormality, particularly the large platelet specimens, the relatively convenient PLT-O method could be used to rectify so as to avoid the pseudothrombocytopenia detected by impedance technology. The relatively complex microscopy could be used if necessary in order to provide more accurate platelet data.
出处
《广州医学院学报》
2012年第5期59-61,共3页
Academic Journal of Guangzhou Medical College
