摘要
采用两步降温法超低温冷冻保存大黄鱼精子,并用透射电镜技术研究了精子的超微结构损伤。结果表明,大黄鱼冻精的激活率、运动时间及寿命与鲜精相比无显著差异。鲜精中28.5%的精子形态结构异常,冻精中37%的精子形态结构异常。形态结构正常的精子表现为质膜与核膜结构完整、无膨胀现象,袖套、轴丝及中心粒结构正常,线粒体形态完整、嵴较发达;形态结构异常的精子表现为质膜破裂、脱落,质膜膨胀,核膜破裂、脱落,核局部受损伤,线粒体膨胀、嵴退化或消失,线粒体移位或脱落。结果显示,以Cortland溶液为稀释液,10%DMSO为抗冻剂,对大黄鱼精子具有较好的抗冻保护作用。
A two-step cooling procedure was employed to cryopreserve Pseudosciaena crocea sperm, and the sperm ultrastructure after which was observed under transmission electron microscopy. The results show that there were no sig- nificant differences between frozen-thawed sperm and fresh sperm by comparing the activation rate, moving time and life-span. Both the fresh sperm and cryopreserved sperm had ultrastructural damages in various degree. The deformation rate of the fresh and cryopreservated sperms were 28.5% and 37%, respectively. The following aspects of cryopreserved sperm with normal morphology was observed： the plasma and nuclear membrane; the sleeve, axoneme and centriole; the mitochondrion obtained integrity and with well-developed cristae. On the contrary, the sperm cryodamages were observed as follows： swelled or disrupted plasma and nuclear membrane; partially damaged nucleus; the swelled, dislocated or dis- articulated mitochondrion with degenerated or vanished cristae. The results show that Cortland solutions and 10% DMSO are the best choice for extender and cryoprotecant, which are helpful for improving the frozen-thawed P. crocea sperm quality.
出处
《海洋与湖沼》
CAS
CSCD
北大核心
2013年第1期56-61,共6页
Oceanologia Et Limnologia Sinica
基金
国家星火计划项目,2011GA701001号
浙江省水产新品种选育专项,2012C12907-8号
宁波市科技计划重大项目,2011C11005号
宁波市科技创新团队项目,2011B82018号
宁波大学优秀学位论文培育基金,PY2012016号
舟山市科技计划项目,10206号
关键词
大黄鱼
精子
超低温冻存
活力
超微结构
Pseudosciaena crocea, Sperm, Cryopreservation, Vitality, Ultrastructure
