摘要
Ti (Ⅳ ) H2 O2 比色法因背景物质干扰而测得的植物叶片内H2 O2 含量偏高 ,5 %三氯乙酸抽提 ,活性炭脱色 ,Ti (Ⅳ ) 4 (2 吡啶偶氮 )间苯二酚 (PAR)比色法测得的H2 O2 含量偏低 .萃取法有效地脱去丙酮提液中的色素 ,且H2 O2 的回收率在 95 %以上 .用过氧化氢酶 (CAT)处理作空白对照 ,利用H2 O2 与Ti (Ⅳ ) PAR的显色反应 ,建立了一种简便、快速、准确的植物叶片内的H2 O2 含量测定方法 ,H2 O2 的最低检测浓度为0 2 5 μmol·L-1.用该方法测得多种植物叶片中H2 O2 的含量在 0 1～ 0
Levels of hydrogen peroxide in plant extracts were overestimated by the method of only using titanium(Ⅳ) because of the interference of pigments and other materials while underestimated by the method of adding activated charcoal(A.C.) in 5% trichloroacetic acid extracts to remove pigments. These problems were avoided by a developed method of extraction, which could not only remove the pigments in acetone extracts conveniently but also get a high recovery more than 95%. Hydrogen peroxide was determined by its reaction with the complex of titanium(Ⅳ)and 4 (2 pyridylazo) resorcinol against references catalase treated. The minimum concentration of hydrogen peroxide determinated in this essay was 0 25 μmol·L -1 . Levels of hydrogen peroxide in leaves of some plant species ranged from 0 1～0 8 μmol·g -1 .
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
2000年第5期548-551,共4页
Progress In Biochemistry and Biophysics
基金
国家自然科学基金!(39770 0 72 ) .
