摘要
高效表达外源蛋白 ,在生物制药中有重要意义 .中国仓鼠卵巢细胞 (Chinesehamsterovarycell)是表达外源蛋白的最佳真核表达系统之一 .影响外源蛋白在CHO细胞表达的因素甚多 ,主要包括载体、宿主细胞和外源基因几方面 .深入了解和灵活运用它们之间的关系 。
It is very important to produce recombinant proteins for therapy in biophar maceutical. Chinese hamster ovary cells(CHO cells) have become one of the best eukaryotic expression systems in recent years. The factors influencing the high level expression of foreign protein in CHO cells and the core strategies of overexpression of recombinant proteins in CHO cells were discussed.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
2000年第5期496-500,共5页
Progress In Biochemistry and Biophysics
基金
国家"8 6 3"计划资助! Z18 0 1
