摘要
组织印迹法（Tissue blotting）是在酶联免疫吸附（Enzyme-Linked immunosorbent Assay ELISA）的基础上发展起来的植物病毒检测技术，该技术不仅保持了ＥＬＩＳＡ对病毒检测的灵敏度高，特异性强的特点，而且大大地简化了操作程序，对病毒的检测更加快速、简单、方便、印迹在硝酸纤维素膜上的样品能保存３个月以上，检测结果能直观地显示出病毒感染的部位。组织印迹技术尤其适用于植物病毒的大规模普查。
Tissue blotting, based on Enzyme-Linked Immunosorbent Assay (ELISA), is a technique for detection of plant viruses. This technique is not only high sensitivity and specificity, but also simpler and more rapid for detection. Samples that are blotted on membrane can be kept over three months. The results can directly display the section of virus infected. It is especially suitable for detection of plant viruses on a large scale.
出处
《微生物学通报》
CAS
CSCD
北大核心
2000年第5期360-363,共4页
Microbiology China
