摘要
将从杉木和大叶黄杨上分离获得的８个胶孢炭疽菌（Colletoctrichum gloeosporioides）分离物培养在含氯酸钾的平板上，得到快速生长抗氯酸钾的不利用硝酸盐的突变体(Nit)。所有的突变体经鉴定分属于３种表现型，即硝酸还原酶结构位点（nit１），硝酸盐同化途径的专化调节位点（nit３），和钼辅因位点（nitM）。分离物发生突变的频率随氯酸钾浓度的增加而提高，并且不同的氮源在一定程度上会影响突变表型种类。除ＣＣ３外，所有的分离物都是自身亲和的，即不同表型的突变体能遗传互补，其中来源于杉木上的两个分离物属于同一个营养体亲和群（ＶＣＧ），其余的分离物属于不同的ＶＣＧ群。
Eight isolates of Colletotrichum gloeosporioides isolated from host plants Cunninghamia lanceolata lanceolata and Euonymus japonichum, respectively, were cultured on MMC medium containing KCIO3 to select chlorate-resistant and nitrate non-utilizing mutants (Nit). All the Nit mutants obtained by this way belong to one of 3 kinds of the following phenotypes: the nitrate reductase structural locus (nit1), the nitrate-assimilation pathway-specific regulatory locus(nit3), and the molybdenum-containing cofactor locus(nitM). The higher mutation frequency on MMC amended with increasing concentration of KCIO3 was induced, and various nitrogen sources were able to influence production of the phenotypic classes. Seven of the 8 isolates tested were self-compatibility in which the two mutants with different phenotypes from the same isolate could genetically complement. The two isolates frail C. lanceolata belong to the same vegetative compatibility group (VCG), the other six isolates belong to distinctive VCGs.
出处
《微生物学通报》
CAS
CSCD
北大核心
2000年第5期341-345,共5页
Microbiology China
关键词
胶孢炭疽菌
不利用硝酸盐突变体
植物病原菌
Colletotrichum gloeosporioides, Nitrate non-utilizing mutants (Nit), Nitrate reductase,Vegetative compatibility group (VCG)€
