摘要
采用水平淀粉凝胶电泳技术对分布于北京金山、檀柘寺、香山、樱桃沟的细距堇菜 (ViolatenuicornisW .Beck .)的遗传变异和群体分化进行了初步研究。对 8个酶系统 11个等位酶位点的检测表明 ,该种在上述地区的遗传变异水平明显高于多年生草本的平均值 ,多态位点百分率P =72 .7% ,等位基因平均数A =2 .4,平均期望杂合度He=0 .2 43 ;群体间的遗传分化略小于多年生草本的平均值 ,杂和性基因多样度的比率Fst=0 .196。检测了空间距离对遗传相似性的影响 ,发现二者无明显的相关性。研究了不同采样方法对遗传学估测的影响 ,表明对该物种的群体进行取样时每群体取 30株时有较充分的代表性 ,小于 2 0个个体的取样可能引起遗传多样性估测出现大的偏差 ,尤其是A和He2个指标易受样本大小的影响。
Genetic diversity and population differentiation of Viola tenuicornis W. Beck. were studied in nine populations in Beijing using horizontal starch gel electrophoresis. Allozyme data for 11 loci of 8 enzymes indicated high level of genetic variability in this region with P =72.7%, A =2.4 and H e =0.243. In contrast, the population differentiation was lower than the average of other perennial herb with F st =0.196. It was found that distances among populations had little effect on population differentiation. A sample of 30 individuals could well represent a population, but less than 20 individuals per population might lead to significant bias in the estimation of genetic parameters, in particular the values of A and H e .
基金
中国科学院生物和技术研究特支费!(财政部专项 ) (STZ_1_10 )&&
关键词
等位酶
细胞堇菜
群体遗传
群传多样性
采样策略
allozyme
genetic diversity
population differentiation
Viola tenuicornis
