摘要
根据新发现的杆状病毒凋亡抑制基因p4 9基因的序列设计了一对引物 ,以SpltNPV基因组DNA为模板 ,通过PCR扩增获得了预期大小的约 1 3kb的DNA片段 ,将此片段克隆到pGEM T载体上并直接测序 .序列分析表明 ,该片段为SpltNPV完整的p4 9基因开放读码框 .与已知的杆状病毒p4 9基因的序列同源性为 87% ,与之对应的氨基酸序列的同源性高达 93% .它是首次从SpltNPV中克隆到的抑制细胞凋亡的基因 .
A fragment of 1\^3 kb was produced by PCR from \%Spodoptera litura\% nucleopolyhedrovirus(\%Spli\%NPV) DNA,with primer,desighed according to the \%Spodoptera littoralis\% nucleopolyhedrovirus(\%Spli\%NPV) \%p49\% gene. The fragment was sequenced after being cloned into the pGEM\|Tvector.The sequencing analysis showed that the fragment was the \%p49\% gene. Compared with \%Spli\%NPV \%p49\% gene,the nucleotide sequence of the fragment was 87% identical and the amino acid sequence deduced from the nucleotide sequence shared 93% identity.
出处
《中山大学学报（自然科学版）》
CAS
CSCD
北大核心
2000年第6期73-76,共4页
Acta Scientiarum Naturalium Universitatis Sunyatseni
基金
国家自然科学基金重点项目资助项目!( 39730 0 30 )
