摘要
将逆行追踪剂荧光金分别注入大鼠一侧臂旁区、延髓尾侧腹外侧区和颈髓第四节段 ,观察到延髓背角浅层 ( 、 层 )向上述部位均有投射 , 层外侧部的投射神经元多于 层内侧部。臂旁区接受双侧延髓背角浅层的投射 ,但以同侧为主 ;延髓尾侧腹外侧区和第 4颈髓接受同侧延髓背角浅层神经元的投射但延髓背角浅层向第 4颈髓投射的神经元数量较少。结合免疫荧光组织化学研究表明 , 层向臂旁区和延髓尾侧腹外侧区投射的神经元部分呈 calbindin-D2 8k样阳性 ,而未见呈 parvalbumin样阳性者。向第 4颈髓投射的 层神经元数量很少 ,未见有呈 calbindin-D2 8K和 parvalbumin样阳性者。本研究结果进一步支持 层有传出投射的观点 ,也提示 层的 calbindin-D2 8K样和 parvalbumin样阳性神经元可能分别属于不同的细胞亚群。
After injecting retrograde tracer fluoro gold(FG) into the parabrachial region(PB), caudal ventrolateral medulla(CVLM) and the fourth segment of cervical spinal cord (C 4), respectively, neurons in laminae Ⅰ～Ⅱ of the medullary dorsal horn projecting to the above mentioned brain areas were observed. PB received projections from bilateral laminae Ⅰ and Ⅱ with an ipsilateral dominance; CVLM and C 4 received projections from ipsilateral laminae Ⅰ and Ⅱ. Neurons projecting to C 4 were very sparsely distributed in laminae Ⅰ and Ⅱ of the medullary dorsal horn. The projecting neurons in outer part of lamina Ⅱ were more than those in inner part of lamina Ⅱ. Combined with immunofluorescence histochemistry for calbindin D28k(CB) and parvalbumin(PV), it was demonstrated that a part of neurons projecting to PB or CVLM showed CB like immunoreactivity, but none of them exhibited PV like immunoreactivity. There were only a few neurons in lamina Ⅱ projecting to C 4 and they exhibited neither CB nor PV like immunoreactivity. The present study provides further evidence for the existence of projecting neurons in lamina Ⅱ and suggests that immunostaining against CB and PV may distinguish two neuronal subpopulations in lamina Ⅱ.
出处
《神经解剖学杂志》
CSCD
北大核心
2000年第3期251-256,共6页
Chinese Journal of Neuroanatomy
基金
国家杰出青年科学基金资助项目!( No. 3 962 5 0 11)
关键词
荧光金
延髓背角Ⅱ层
PB
CVLM
C4
投射神经元
fluorogold
calbindin D28k
parvalbumin
medullary dorsal horn
lamina Ⅱ
parabrachial region
caudal ventrolateral medulla
cervical cord
rat
