摘要
目的探讨K-ras蛋白表达、基因突变在皮肤瘢痕癌组织中的意义。方法以皮肤病理性瘢痕、皮肤瘢痕癌组织为研究对象,以正常皮肤组织为对照。采用免疫组织化学(SP)法检测K-ras蛋白的表达。另外,从瘢痕和瘢痕癌石蜡组织中提取DNA,进行PCR扩增及测序,分析K-RAS基因第12、13位密码子点突变情况。所有数据运用SPSS16.0软件包进行统计学分析。结果①K-ras蛋白在正常皮肤表皮和皮肤病理性瘢痕上皮中呈弱阳性表达,在瘢痕癌组织中呈强阳性表达。瘢痕癌组的表达与正常皮肤组及皮肤瘢痕组比较,差异均有显著性(P<0.05);正常皮肤组与瘢痕组比较,差异无显著性(P>0.05)。②从14例皮肤瘢痕和14例瘢痕癌石蜡组织中提取DNA,经PCR反应,均成功扩增出目的片段,测序未发现K-ras基因第12、13位密码子的点突变。结论①皮肤瘢痕癌的发生可能与K-ras蛋白的高表达有关。②瘢痕癌中K-ras基因的突变位点有待进一步探讨。
[ Objective ] To investigate the association of K-ras protein expressions and gene mutations with skin scar carcinoma tissues. [ Methods ] The skin pathological scar and skin scar carcinoma tissues were compared with normal skin tissues. The expression of K-ras protein was detected by the immunohistochemical method of SP. Be- sides, DNA was extracted through choose 14 skin pathological scar tissues and 14 scar carcinoma tissues fixed in 10% formalin and Paraffin embedded （28 samples）. K-ras mutations in codons 12 and 13 were analyzed using PCR and sequencing. All data was input into the computer and statistically analyzed with SPSS （16.0） software. [ Results ] （1）K-ras showed strong positive expression in scar carcinoma tissues, weakly positive expression in normal skin epi- dermis and skin pathological scar epithelium. Compared with normal skin group and skin scar group, the expression of K-ras in scar carcinoma group were statistically significant （P 〈0.05）. No statistic differences of their expression were measured between normal skin group and skin pathological scar group （P 〉0.05）. （2）DNA was extracted through the chosen 14 skin pathological scar tissues and 14 scar carcinoma tissues fixed in paraffin embedded. Using PCR, the 28 samples had been amplified the K-ras gene. The K-ras gene in codons 12 and 13 were not examined to have mutation after sequencing. [ Conclusions] （1）The high expression of K-ras protein may be related to the occurrence of skin scar carcinoma. （2）The situs of K-ras mutation in skin scar carcinoma is to be further explored.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2013年第1期22-25,共4页
China Journal of Modern Medicine
基金
贵州省攻关项目(No:2010-3080)
