摘要
目的检测复发性自然流产（recurrentspontaneousabortion，RSA）孕妇外周血及其龈沟液中自介素4（interleukin-4，IL-4）、干扰素γ（interferon-γ，IFN-γ）的含量并进行比较，探讨牙周炎是否会引起体液免疫失调，引起孕妇宫腔内免疫环境向T辅助细胞1型（T-helper1，Th1）漂移并引发流产。方法检查RSA孕妇50例（孕5至28周）（RSA组）和足月待产孕妇40例（孕39至41周）（对照组）的牙周状况，收集其龈沟液和静脉血，对其进行口腔卫生宣教，酶联免疫吸附测定法检测龈沟液和静脉血中IFN-γIL-4的水平。结果RSA组9例患牙周炎，患病率为18％（9／50），对照组5例患牙周炎，患病率为13％（5／40），两组牙周炎的患病率差异有统计学意义（P〈0．05）；RSA组的探诊深度、附着丧失及出血指数均显著高于对照组（P〈0．05）。RSA组孕妇龈沟液及静脉血中IFN．1含量[分别为（52．98±17．56）、（27．79±3．59）ng／L]均显著高于对照组[分别为（25．254±7．93）、（18．39±2．65）ng／L]，P〈0．05；RSA组孕妇龈沟液及静脉血中IL-4的含量[分别为（15．43±1．77）、（15．88±0．95）ng／L]均显著低于对照组[（19．62±4．04）、（22．98±4．30）ng／L]，P〈0．01。相同组内静脉血与龈沟液中IFN-γ、IL-4的含量均呈正相关关系（r值均〉0．8，P〈0．001）。结论RSA孕妇患牙周炎的几率较高，牙周组织中IFN-γ、IL-4浓度的改变会进一步引发静脉血中IFN-γ、IL-4的浓度改变，使Th1-Th2平衡向Th1漂移；RSA孕妇与其患牙周炎存在相关性。
Objective To investigate the relationship between recurrent spontaneous abortion （RSA） and the level of interferon-γ （IFN-γ ） and interleukin-4 （IL-4） in peripheral blood and gingival crevicular fluid of patients with chronic periodontitis. Methods Fifty pregnant women with recurrent spontaneous abortion（ gestational age ： 5-28 weeks） and 40 pregnant women （ gestational age ： 39-41 weeks） were included and their periodontal status was examined. The level of IFN-γ and IL-4 in gingival crevicular fluid and peripheral blood was detected by enzyme-linked immunosorbent assay. Results Nine RSA women and 5 in control group had periodontitis. The prevalence of periodontitis in RSA group was 18% （9/50） and 13% （5/40） in the control group（ P 〈 0. 05 ）. The probing depth, attachment loss and bleeding index in RSA group were higher than those in the control group（P 〈0. 05）. The level of IFN-γ was （52. 98 ± 17. 56） ng/ L in gingival crevicular fluid of RSA group and （25.25 ±7.93） ng/L in control group（P 〈0. 01 ）. The level of IL-4 was （ 15.43 ± 1.77 ） ng/L in gingival crevicular fluid of RSA group and （ 19. 62 ± 4.04） ng/L in control group （ P 〈 0. 01 ）. The content of IFN-γwas （27.79 ± 3.59） ng/L in peripheral blood of RSA group and （ 18.39± 2. 65 ） ng/L in control group（P 〈 0.05 ） . The content of IL-4 was （ 15.88 ± 0. 95 ） ng/L in peripheral blood of RSA group and （ 22. 98 ± 4. 30 ） ng/L in control group （ P 〈 0. 001 ）. The content of eytokines in gingival erevicular fluid and peripheral blood in the same group was positively related （ r 〉 0. 8, P 〈 0. 001 ）. Conclusions Pregnant women of RSA have a higher chance of getting periodontitis. The change in the level of eytokines in periodontal tissue could result in excursion of maternal intrauterineimmune environment to T-helper 1 （ Thl ）. Periodontitis was related to RSA.
出处
《中华口腔医学杂志》
CAS
CSCD
北大核心
2013年第3期150-154,共5页
Chinese Journal of Stomatology
