摘要
目的 检测急性和慢性再生障碍性贫血 (再障 )患者骨髓单个核细胞 (BMMNC)培养上清白细胞介素 4(IL 4)、γ干扰素 (IFN γ)及肿瘤坏死因子α(TNF α)的诱生水平 ,了解两组患者免疫学状况的异同点。方法 利用双抗体夹心ELISA法测定BMMNC培养上清中IL 4、IFN γ和TNF α的诱生水平 ,比较两组患者上述细胞因子水平的差异。结果 ①所有患者IFN γ、TNF α水平均增高 ,与对照组比较 ,差异有显著性。急性组IL 4大致正常 ,与对照组比较 ,差异无显著性 ;慢性组IL 4水平升高 ,与对照组比较 ,差异有显著性。②在急、慢性组之间 ,TNF α水平差异无显著性 ;但IL 4、IFN γ水平差异有显著性。结论 异常细胞免疫效应可能在急性再障发病中起主要作用 ;慢性再障既可能有细胞免疫增强 ,又有体液免疫功能亢进。
Objective To detect the induced levels of IL-4,IFN-γ and TNF-α in the supernatant of bone marrow mononuclear cells (BMMNC) and compare the difference of immune status between acute(SAA) and chronic(CAA) aplastic anemia patients. Methods Concentrations of IL-4、IFN-γ and TNF-α in PHA-P-induced BMMNC supernatants were determined by ELISA assay in 11 SAA and 13 CAA patients as well as 16 controls. Concentration differences between the two groups were compared. Results ① The IFN-γ and TNF-α levels in AA patients studied were much higher than that in controls,and IL-4 levels were normal in SAA group but elevated in CAA group. ② TNF-α levels were comparable between the two AA groups, but both IL-4 and IFN-γ levels were significantly different between them. Conclusion Enhanced cellular immunity seems to play an important role in the pathogenesis of SAA, and enhancement of both cellular and humoral immunity might contribute to the pathogenesis of CAA.
出处
《中华血液学杂志》
CSCD
北大核心
2000年第10期530-532,共3页
Chinese Journal of Hematology
基金
国家自然科学基金!资助项目 (3990 0 0 6 2 )
