摘要
目的 系统观察窦内皮细胞 (SEC)及其基膜中Ⅳ型胶原 (CoⅣ )及层黏素 (LM )在人慢性肝病和肝细胞癌 (HCC)的病理形态学改变 ,并探讨其临床病理意义。方法 对 30例人HCC及相应癌旁组织 ,10例肝硬化 ,5例轻度慢性肝炎和 4例正常肝组织进行了CD34、CoⅣ和LM的免疫组化检测 ,行半定量计数 ,系统地观察SEC及基膜成份在正常、慢性肝病和肝癌中的形态和数量的改变 ,并结合肝癌的临床病理资料进行分析。结果 正常肝窦无CD34及LM的表达 ,CoⅣ沿窦壁呈断续弱阳性表达 ,慢性肝病时可有少量CD34及LM弱阳性 ,肝硬化时CoⅣ阳性增强 ,沿窦壁呈均匀的连续线状阳性。HCC时窦壁呈弥漫的CD34阳性 ,同时CoⅣ及LM的阳性表达明显增强 ,在低分化癌及有门静脉癌栓的癌组织中基膜表达有所减少 ,易见断裂及缺失。LM、CoⅣ ,尤其是CD34的表达在癌组织与非癌性肝组织的表达差异有显著性。临床病理资料分析显示 ,瘤径 <2cm者CD34表达显著低于瘤径 >5cm者 ,CoⅣ的表达在HCC低分化组明显减少 ,有门静脉癌栓组CoⅣ和LM表达均有减少。结论 CD34是鉴别肝癌组织与非癌性肝组织的可靠指标。肝癌中基膜的断裂。
Objective To observe the change of sinusoid lining endothelium cell(SEC), type Ⅳ collagen (CoⅣ) and laminin (LM) in chronic liver disease and hepatocellular carcinoma(HCC), and its clinicopathologic significance in HCC. Methods 30 cases of HCC and corresponding noncancerous tissues, 10 cases of liver cirrhosis, 5 cases of mild chronic hepatitis and 4 cases of normal liver tissues were stained with CD34, CoⅣ and LM by immunohistochemistry. Semi quantitation of CD34, CoⅣ and LM were studied and analyzed with clinical and pathologic features of HCC. Results There was no expression CD34 and LM along sinusoidal walls in normal human liver, while CoⅣ was weakly and discontinuously expresssion. In liver cirrhosis, sinusoidal positive expression of CoⅣ increased significantly and became continuous and homogeneous. CD34 and LM were shown in the perinodule with marked inflammatory cells infiltration. HCC revealed a diffuse capillarization with CD34, CoⅣ and LM expression increase. CoⅣ and LM expression were decreased in poorly differentiated tumors and tumors with tumor thrombus, mostly accompanied by break and loss of basement membrane. CD34 expression of tumor size<2 cm was significant lower than that of tumor size>5 cm. The expression of CD34 and LM were markedly increased in HCC compared with noncancerous liver tissues. Conclusion HCC shows a diffuse capillarization with CD34, CoⅣ and LM expression increase. The frequent break, loss and decrease of basement membrane in poorly differentiated tumors and tumors with portal vein infiltration may suggest that they may play a major role in the metastasis of HCC.CD34 could be a useful marker for distinguishing HCC from non cancerous liver tissues.
出处
《中华消化杂志》
CAS
CSCD
北大核心
2000年第5期311-314,共4页
Chinese Journal of Digestion
关键词
肝癌
CD34
Ⅳ型胶原
窦壁内皮细胞
病理形态学
Hepatocellular carcinoma
Chronic liver disease
CD34
CoⅣ
Laminin
Capillarization
