摘要
目的对肝尔舒复方分离纯化所得4个部位进行保肝降酶活性评价,确定其有效部位。方法肝尔舒复方水提液经大孔树脂、阳离子树脂、阴离子树脂纯化后得到总黄酮和总皂苷、总生物碱、总有机酸。0.2%四氯化碳溶液制备小鼠肝损伤模型,给予以上分离所得4个部位及其混合物,测定小鼠血清中丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)及肝组织中超氧化物歧化酶(SOD)活性及丙二醛(MDA)水平。结果肝尔舒复方有效部位混合组和总黄酮皂苷组可明显抑制急性肝损伤小鼠血清中ALT、AST活性(P<0.01),总生物碱组和总有机酸组也可以一定程度抑制急性肝损伤小鼠血清中ALT、AST活性(P<0.05),以及肝组织中SOD活性,降低MDA水平(P<0.05);阳性对照组和混合组总胆红素(T-BiL)及肝系数显著降低(P<0.01或P<0.05)。结论肝尔舒复方4个有效部位均有不同程度的保肝降酶功效,其中有效部位混合物相对于其他各有效部位作用更明显。
Objective To evaluate the effects of four fractions isolated from ganershu compound on protecting liver and lowering transaminase. Methods Total flavonoids, saponins, alkaloids and organic acids were purified from water extracts of ganershu compound by macroporous resin, cationic resin, and anionic resin. Each of the four fractions and the mixture were given to mice with liver injury induced by 0.2% carbon tetrachloride （ CC14 ）. The alanine aminotransferase （ALT） and aspartate ami- notransferase （AST） in the mouse serum were detected, and both superoxide dismutase （SOD） and malondialdehyde （MDA） in the hepatic tissue were tested. Results The four-fraction mixture, the total flavonoids and the saponins significantly inhibited the activity of serum ALT, AST in mice with acute liver injury （P〈0.01）. The total alkaloids and total organic acids also de- creased the activity of AST, ALT （P〈0.05） and SOD, and reduced the content of MDA （ P〈0.05 ）. The total bilirubin （ T- BiL） and liver index in the positive control and mixture groups were markedly decreased （P〈0.01 and P〈0.05, respectively） as compared to the total alkaloids and total organic acids groups. Conclusion The four effective fractions of ganershu compound extract provide liver protection and transaminase reduction to different extents, with the mixture showing more pronounced effects.
出处
《医药导报》
CAS
北大核心
2013年第3期285-288,共4页
Herald of Medicine
基金
江苏省科技支撑计划项目(BE2009682)
关键词
肝尔舒复方
保肝
降酶
有效部位
Ganershu compound
Liver protection
Transaminase reduction
Effective fractions
