摘要
目的探讨白子菜水提液对胰岛素抵抗HepG2细胞关键酶活性的影响。方法采用葡萄糖临床检测试剂盒、肝糖原测定试剂盒检测白子菜水提液对胰岛素抵抗HepG2细胞葡萄糖消耗和HepG2细胞的糖原含量的影响;采用葡萄糖-6-磷酸脱氢酶耦联比色法、乳酸脱氢酶耦联比色法及钼酸铵定磷法测定葡萄糖激酶(GK)、磷酸烯醇式丙酮酸羧激酶(PEPCK)和葡萄糖-6-磷酸酶(G-6-Pase)的活性。结果白子菜水提液能够促进胰岛素抵抗HepG2细胞的葡萄糖消耗,使G-6-Pase及PEPCK活性分别降低71.41%,82.14%,使GK活性和糖原含量分别提高28.77%,96.73%。结论白子菜水提液可降低胰岛素抵抗HepG2细胞G-6-Pase和PEPCK的活性,抑制糖异生作用,从而减少细胞内源性葡萄糖的产生。此外,白子菜水提液还提高GK活性,加快糖酵解的进行,增加糖原含量,减轻HepG2细胞的胰岛素抵抗状态。
Objective To explore the effect of aqueous extracts from shirako on key enzyme activity in insulin-resistant HepG2 cells. Methods Glucose consumption and glycogen content of insulin-resistant HepG2 were detected by glucose clinic test kit and hepatic glycogen test kit after treatment with water extracts of shirako. Activities of glucokinase （ GK）, phosphoenol- pyruvate carboxylase kinase （PEPCK）, and glucose-6-phosphatase （G-6-Pase） were assayed by the glucose 6-phosphate dehy- drogenase coupling colorimetric, lactate dehydrogenase coupling eolorimetric and ammonium molybdate constant phosphorus meth- ods. Results The aqueous extracts of shirako enhanced glucose consumption, lowered the activity of G-6-Pase and PEPCK （71.41% ,82.14% ）, increased the activity of GK and glyeogen content of insulin-resistant HepG2 （28.77% ,96.73% ）. Conclusion Aqueous extracts of shirako plays a role in lowering PEPCK and G-6-Pase activities and inhibiting glucogenesis, re- sulting in the reduction of endogenous glucose in the cell. In addition, it also can augment the activity of GK, accelerate the process of glucolysis, increase the glycogen content, and alleviate insulin resistance of HepG2.
出处
《医药导报》
CAS
北大核心
2013年第3期281-284,共4页
Herald of Medicine
基金
广西科技基础条件平台建设项目"中药药效研究重点实验室"(桂科能0907006)
