摘要
以纳米TiO2为基底,L-色氨酸(L-Trp)为模板分子,采用溶胶凝胶法合成L-Trp印迹的纳米TiO2。采用扫描电镜和红外光谱对印迹纳米TiO2和非印迹纳米TiO2进行表征,表明模板分子L-Trp成功地印迹到纳米TiO2中。采用1%的氨水溶液去除印迹的纳米TiO2中的模板分子L-Trp,纳米TiO2上留下与L-Trp相匹配的空穴。采用荧光分光光度法研究了具有L-Trp铸型的纳米TiO2对L-Trp和D-色氨酸(D-Trp)的分离效果。实验表明,模板分子L-Trp与钛前驱体的最佳投料摩尔比为1∶0.2,富集溶液pH值为6.0时,印迹纳米TiO2对L-Trp具有优异的选择吸附性,其分离选择系数为2.42,可实现对色氨酸对映体的识别。
A novel imprinted TiO_2 nanoparticles were synthesized via a sol - gel process using L-tryptophan（L-Trp） as temple molecules and TiO_2 nanoparticles as imprinted matrix. The imprinted TiO_2 nanoparticles were investigated by scanning electron microscopy and infrared spectroscopy. The result indicated that L-Trp was imprinted into TiO_2 nanoparticles successfully. Appropriate template cavities in TiO_2 nanoparticles were formed after the template molecules were removed by treatment with 1% ammonia solution. Fluorescence spectrophotometry was used to study the separation abilities of L-Trp and D-tryptophan （D-Trp） in the imprinted TiO_2 nanoparticles. The results showed that imprinted TiO_2 nanoparticles had a high selectivity to L-Trp, and the separation factor was 2.42 under the optimum molar ratio of L-Trp to titanium precursor（ 1 ： 0.2） in pH 6. 0 solution.
出处
《分析测试学报》
CAS
CSCD
北大核心
2013年第3期331-335,共5页
Journal of Instrumental Analysis
基金
国家自然科学基金资助项目(21175016
21105006)
关键词
溶胶凝胶
分子印迹
色氨酸
纳米TiO_2
sol - gel
molecular imprinting
L-tryptophan
titanium dioxide nanoparticles
