摘要
探讨煤烟导致细胞体系氧化应激损伤的机制。 [方法 ]以N 乙酰半胱氨酸 (NAC)和甘露醇等不同作用位点的抗氧化剂作为实验干预手段 ,采用MTT比色法和溴乙锭荧光法观察煤烟所致细胞毒性和肺细胞DNA交联形成作用。 [结果 ]N 乙酰半胱氨酸 (NAC)可以有效地降低煤烟所致的细胞毒性和细胞DNA交联形成作用 ;而甘露醇却没有观察到上述作用。 [结论 ]煤烟在细胞内经代谢活化后产生的某些代谢产物可以降低体内谷胱甘肽含量 ,引起细胞DNA的氧化应激损伤。
Objective] This study is to explore the mechanism of oxidative stress induced by soot in cells. [Method] Before exposure to soot extracts,the rat pulmonary cells were pretreated with anti oxidative agents mannitol or N acetylcysteine (NAC) for 30 minutes,then they were exposed to the soot particulate extracts for 24 hours. MTT assays and ethidium bromide fluorescence assays (EFA) were applied to detect the cytotoxicity and DNA cross links. [Results] It is shown that NAC could reduce the cytotoxicity and DNA cross links induced by soot in rat pulmonary cells,but mannitol could not. [Conclusion] It suggested that soot can induce the oxidative stress in the rat pulmonary cells,it may be due to the reduced levels of GSH in cells.
基金
国家自然科学基金资助课题! (3940 0 0 30 )
关键词
煤烟
自由基
抗氧化剂
氧化应激损伤
甘露醇
soot
rat pulmonary cell
oxidative stress
cytotoxicity
mannitol N acetylcysteine
