摘要
采用盆栽及室内模拟实验对铝胁迫下大豆根际微生物的群体感应进行了初步探索。利用高效检测菌株根癌农杆菌Agrobaoterium tumefaciens KYC55(pJZ372;pJZ384;pJZ410)对在酸性土壤中种植了耐铝大豆BX10和铝敏感大豆BD2的根际土、非根际土中群体感应信号分子高丝氨酸酰基内酯(AHL)进行检测。结果表明,在大豆根际土中AHL活性显著高于非根际土,且BX10根际土中AHL含量显著高于BD2(P<0.01);随大豆生长时期的推移,BX10和BD2根际土及非根际土中AHL均逐渐升高。模拟酸铝环境的室内实验表明,不同浓度的铝胁迫对中华根瘤菌Sinorhizobium sp.1128的生长、AHL产生水平、胞外多糖的产生以及根毛吸附作用均产生显著影响,从而进一步验证了铝胁迫对群体感应的影响。
Bacterial quorum sensing （Qs） in the rhizosphere soil of soybean in acid soil was studied with pot and laboratory experiments. N - acyl - homoserine lactone （AHL） was one of QS signal molecules, it was extracted from the rhizosphere and bulk soils of aluminum （A1）-tolerant （BXI0） and A1-sensitive （BD2） soybean geno- types cultured in acid soil , and was detected by Agrobacterium tumefaciens KYC55 （pJZ372; pJZ384; pJZAI0）. The results showed that the AHL can be detected in the rhizosphere soil of soybeans ; the AHLs activities in the rhi-zosphere soil were much higher than those in the bulk soil; the AHLs activities in the rhizosphere soil of BXI0 were much higher than those of BD2 （P〈0.01）. Moreover, AHLs increased gradually during the process of soybean growth. The results of the laboratory experiments simulating the acid soil environment showed that different concen- trations of A1 significantly influenced tile growth, the AHLs level, the production of exopolysaccharide and root hair adherence of Sinorhizobium sp. 1128, which further demonstrated the effects of aluminum stress on QS. This study facilitates the further studies about the molecular mechanism of interaction between plant and rhizosphere microbes in acid soils.
出处
《中国油料作物学报》
CAS
CSCD
北大核心
2013年第1期74-79,共6页
Chinese Journal of Oil Crop Sciences
基金
国家自然科学基金(30971871
40771107)
教育部创新团队项目(IRT1020)
教育部高校科技创新工程重大项目培育资金(707027)
关键词
大豆
酸性土壤
铝毒
根际微生物
群体感应
AHL
Soybean
Acid soil
Aluminum toxicity
Rhizosphere microbial
Quorum sensing
AHL
