摘要
从甘肃省河西走廊改良后的盐碱地分离链霉菌用于油菜菌核病菌的拮抗研究,并对拮抗菌进行鉴定。分离得到10株不同菌落形态的链霉菌,其中有2菌株对油菜菌核病有拮抗作用,编号Ⅳ22-3-3和Ⅳ22-3-12。与油菜菌核病进行对峙培养结果发现,Ⅳ22-3-3和Ⅳ22-3-12抑菌圈直径分别为1.2和0.9cm;离体叶菌丝接种试验表明,Ⅳ22-3-3和Ⅳ22-3-12对油菜菌核病菌的防治效果分别达63.5%和49.1%;Ⅳ22-3-12能在油菜菌核上定殖并寄生分解菌核,同对照相比菌核萌发率下降18.2%;通过形态培养特征、生理生化特征和16SrDNA鉴定表明,Ⅳ22-3-3为产水链霉菌(Streptomyces hydrogenans),Ⅳ22-3-12为球孢链霉菌(Streptomyces globisporus)。
Different Streptomyces strains were isolated from saline - alkali soils of Hexi Corridor in Gansu Province. Among them, two strains named IV22-3-3 and 1V22-3-12 were found to be antagonists inhibiting Sclerotinia sclerotiorum isolated from oilseed rape. Detached leaf inoculation tests showed that the two strains had preventing effects of 63.5% and 49.1% respectively. Moreover, IV22-3-12 could colonize on the sclerotia and inhibit the germination of the pathogen. Compared to physiological saline （control）, IV22-3-12 decreased 18.2% of the sclerotia germination rate. Morphological and physiological-biochemical characteristics as well as phylogenetic analysis based on 16S rRNA gene sequence supported strain 1V22-3-3 to be Streptomyces hydogenans, and strain IV22-3-12 to be Streptomyces globisporus.
出处
《中国油料作物学报》
CAS
CSCD
北大核心
2013年第1期69-73,共5页
Chinese Journal of Oil Crop Sciences
基金
国家自然科学基金(31260134
30960078)
2009年度人事部留学人员科技活动择优项目
关键词
油菜
核盘菌
菌核
盐碱地
链霉菌
生物防治
Rapeseed
Sclerotinia sclerotiorum
Sclerotia
Saline - alkali soils
Streptomyces sp.
Biological control
