摘要
利用 Yop1- Mc Ab- RIHA(反向间接血球凝集试验 )检测全国 17个生态型 10 5株鼠疫耶氏菌 (Yersinia pestis)强毒株和 4株弱毒标准株、6株不同血清型的假结核耶氏菌、4株小肠结肠炎耶氏菌以及 3株其它肠道菌。结果显示 ,假结核和小肠结肠炎耶氏菌呈现较高滴度的阳性反应 (滴度 1∶ 2 7～ 1∶ 2 1 0 ) ,其余均为阴性 ,提示 :(1) Yop1蛋白为耶尔森氏菌所特有 ;(2 )鼠疫耶氏菌中突变的 yop A基因发生回复突变的可能性不大 ,进一步的结论将在扩大检测后作出 ;(3)据此 ,有理由认为 Yop1蛋白单克隆抗体及其检测技术在鼠疫与假结核耶氏菌及小肠结肠炎耶氏菌 O∶ 9血清型与布鲁氏菌的鉴别诊断上有良好的应用前景。
The 105 strains with high virulence of Yersinia pestis from 17 ecotype, 4 strains with low virulence, preserved by our laboratory, 6 Yersinia pseudotuberculosis strains, 4 Yersinia enterocolitica strains and 3 strains of the other intestinal canal bacteria were t[WT5,5”BZ]ested by Yop1 McAb RIHA. While the strains of Y.pseudotuberculosis and Y.enterocolitica appeared positive reaction (dilution rate:2 7~10 ),negative reaction were produced in the rest strains. These suggested:(1) Yop1 be the exclusive protein of Yersinia spp.; (2)The possibility of reverse mutation of yopA gene in Yersinia pestis be very little (the detailed research will report in another essay); (3)The Yop1 McAb and its immune measure techniques have more widespread prospects in the differential diagnosis between the Yersinia pestis[WT5,5”BZ] and Yersinia pseudotuberculosis [WT5,5”BZ]and between the Yersinia enterocolitica serotype O∶9 and Brucilla spp.
出处
《地方病通报》
2000年第3期1-2,共2页
Endemic Diseases Bulletin
基金
国家自然科学基金
新疆自然科学基金!(391 60 0 70)
