摘要
目的 :进一步了解端粒酶活性和端粒酶 RNA组分 (h TR)与喉癌发生、发展的关系 ,并建立喉癌的早期诊断标准。方法 :采用端粒酶重复扩增法和原位杂交法对正常组织、声带息肉、癌前病变和喉癌进行 h TR和端粒酶活性分析。结果 :1所有组织均表达不同水平的 h TR,h TR阳性率随着喉癌的临床分期逐渐增加 ,但无统计学差异。 2 39例 (78% )的喉癌组织表达端粒酶活性 ,声带息肉仅 1例 (8.3% )表达 ,不同分化的喉癌组织端粒酶活性有差异。结论 :端粒酶活化是喉癌发生的早期事件 ,检测端粒酶活性可判定临床喉部肿瘤的良恶性。但 h TR与端粒酶表达不一致 ,不能作为预测端粒酶活性的指标。
Objective:Expression of telomerase activity and telomerase RNA component have been reported in various human cancers.In the article,we ascertained whether telomerase activity and hTR changes are charateristic of laryngeal cancer.Method:Telomerase activity were examined by telomeric repeat amplification protocol(Trap) and hTR examined by in situ hybridization,in tissue samples from 50 laryngeal cancer,12 laryngeal polyps,14 precarcinous,9 normal laryngeal tissue.Result:①All tissue expressed various levels of hTR.Positive rate of hTR increased with the clinical stage,but no significant correlation was observed,as well as lymph node metastasis.②The telomerase activity were detected in samples 39 of the 50 laryngeal cancer(78%).However,in 12 cases of begin lesions only 8.3 %(1/12).Telomerase activity was found to have a relationship with the grade of tumor differentiation.Poorly differentiated laryngeal cancer expressed high level telomerase activity.Conclusion:Telomerase activity were an early event of laryngeal cancer.It suggests that detection of telomerase activity could be a good diagnostic marker for distinguish cancer from normal cell.
出处
《临床耳鼻咽喉科杂志》
CAS
CSCD
2000年第9期390-392,共3页
Journal of Clinical Otorhinolaryngology
关键词
端粒酶活性
喉癌
端粒酶重复扩增法
RNA组分
Telomerase activity Laryngeal neoplasms Telomeric repeat amplification protocol
