摘要
目的建立测定肾石消胶囊中大黄素含量的高效液相色谱法。方法以Thermo BDS Hypersil C18柱(250 mm×4.6 mm,5μm)为固定相,甲醇-0.1%磷酸溶液(85∶15)为流动相,检测波长为254 nm,流速为1.0 mL/min,柱温为室温。结果大黄素进样量在0.265 5～5.31μg范围内与峰面积线性关系良好,r=0.999 9,平均回收率为99.43%,RSD为0.47%(n=6)。结论该方法快速、简单、准确、可用于该制剂中大黄素的含量测定。
Objective The aim was to determine emodin content in Shenshixiao Capsules by HPLC. Methods The Thermno BDS HY- PERSIL C18 column（250 mm×4.6 mm,5μm）） was used,the mobile phase was methanol- 0. l%phosphoric acid（85：lS）, the detecting wavelength was 254 nm,the flow velocity was 1.0 mL/min,column temperature was room temperature. Results The linear relationship of emodin was good within the range of0.265 5～5.31μg（r=0.999 9）,the average recovery was 99.43%, RSD was 0.47% （n=6）. Conclusion The method is rapid,accurate and simple,it can be used to determine the content of emodin in shenshixiao Capsules.
出处
《中国药业》
CAS
2013年第5期49-50,共2页
China Pharmaceuticals
