摘要
目的探讨白细胞介素6／信号转导及转录激活因子3（IL-6／STAT3），信号通路在脓毒症大鼠肺组织高迁移率族蛋白B1（HMGB1）表达上调中的作用。方法健康雄性Wistar大鼠90只．10～14周龄，体重250～300g，采用随机数字表法，将其随机分为3组（n=30）：假手术组（s组）、脓毒症组（CLP组）和抗IL-6单克隆抗体组（IL-6组）。采用盲肠结扎穿孔法制备大鼠脓毒症模型。IL-6组于术前1h时腹腔注射抗IL-6单克隆抗体0．5mg／kg，S组和CLP组给予等容量生理盐水。于术后6、12、24、48、72h时分别处死大鼠6只，取肺组织，分别采用酶联免疫吸附法和实时荧光定量聚合酶链反应法检测肺组织HMGBl含量和HMGB1mRNA表达；采用凝胶阻滞电泳分析技术检测肺组织STAT3DNA结合活性。结果与s组比较，CLP组和IL-6组肺组织HMGB1含量和HMGB1mRNA表达、STAT3DNA结合活性升高（P〈0．05）；与CLP组比较，IL-6组肺组织HMGBl含量和HMGB1mRNA表达、STA13DNA结合活性降低（P〈O．05）。结论IL-6／STAT3信号通路参与了脓毒症大鼠肺组织HMGB1表达上调。
Objective To evaluate the role of interlukin-6 and signal transducer and activator of transcription 3 （IL-6/STAT3） signaling pathway in the up-regulation of high mobility group box 1 （HMGB1） expression in lung tissues in septic rats. Methods Ninety healthy male Wistar rats, aged 10-14 weeks, weighing 250-300 g, were randomly divided into 3 groups （n = 30 each） ： sham operation group （group S）, cecal ligation and puncture （cLP） group and anti-IL-6 monoclonal antibody group （group 1L-6）. Sepsis was induced by CLP. Anti-IL-6 mon-oclonal antibody 0.5 mg/kg was injected intraperitoneally 1 h before CLP, while the equal volume of normal saline was given in groups S and CLP. Six rats in each group were sacrificed at 6, 12, 24, 48 and 72 h after CLP and lungs were removed for determination of HMGB1 content （by RT-PCR）, HMGB1 mRNA expression （ by ELISA） and STAT3-DNA binding activity （by electrophoretic mobility shift assay） in lung tissues.Results Compared with S group, HMGBI content, HMGB1 mRNA expression and STAT3-DNA binding activity were significantly increased in groups CLP and IL-6 （P 〈 0.05）. HMGBI content, HMGB1 mRNA expression and STAT3-DNA bind- ing activity were significantly lower in group IL-6 than in group CLP （ P 〈 0.05）. Conclusion IL-6/STAT3 signaling pathway is involved in the up-regulation of HMGB1 expression in lung tissues in septic rats.
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2013年第1期88-90,共3页
Chinese Journal of Anesthesiology
