摘要
目的:观察氯胺酮对强迫游泳大鼠前额皮层谷氨酸及γ-氨基丁酸的影响。方法:32只Wistar雄性大鼠随机均分为4组(n=8):生理盐水0.5h组(S0.5组)、生理盐水2h组(S2组)、氯胺酮0.5h组(K0.5组)、氯胺酮2h组(K2组)。药物干预前1d大鼠行强迫游泳15min制备急性应激抑郁模型。药物干预当天,给大鼠分别腹腔注射1mL容积的生理盐水或氯胺酮10mg/kg,给药后0.5h及2h分别再次行强迫游泳6min,记录后5min内不动时间。行为学测试结束后,取大鼠前额皮层,检测谷氨酸及γ-氨基丁酸的含量。结果:与S0.5组相比,K0.5组大鼠强迫游泳不动时间显著减少,谷氨酸含量上升,γ-氨基丁酸含量下降(P<0.05)。与S2组相比,K2组大鼠强迫游泳不动时间显著降低(P<0.05),谷氨酸及γ-氨基丁酸含量则无明显变化(P>0.05)。结论:氯胺酮在大鼠强迫游泳模型中能够发挥快速抗抑郁作用,同时伴随大鼠前额皮层谷氨酸上调及γ-氨基丁酸下调。
Objective To observe the effects of ketamine on glutamate and GABA in prefrontal cortex of rats receiving forced swimming test （FST）. Methods 32 male Wistar rats were randomized into 4 groups （each n = 8） ： saline 0.5 h group （S0.5 group）, saline 2 h group （S2 group）, ketamine 0.5 h group （K0.5 group）, ketamine 2 h group （K2 group）. The FST lasted for 15 min was used to establish rat depression model. On the next day, saline or ketamine （10.0 mg/kg） of 1mL were intraperitoneally injected, then 0.5 h and 2 h later, the FST of 6 min was carried out and the immobility time within 5 rain during the FST was recorded. The animals were then decapitated and the prefrontal cortex was harvested for determination of glutamate and γ-aminobutyric acid levels. Results Compared with those in S0.5 group, the immobility time was decreased, the content of glutamate was increased and the euntent of γ-aminobutyric ac.id was decreased signifieantly in K0.5 group （P 〈 0.05）. Compared with those in S2 group, the immobility time was decreased significantly （P 〈 0.05）, the content of glutamate and γ-aminobutyric acid had no significant changes in K2 group （P 〉 0.05）. Conclusion Ketamine possessed significant and rapid antidepressant effects in rats receiving FST, which was associated with the increase of glutamate and the decrease of γ-aminobutyrie acid levels in prefrontal cortex.
出处
《实用医学杂志》
CAS
北大核心
2013年第6期877-879,共3页
The Journal of Practical Medicine
基金
国家自然科学基金(编号:30872424)
全军"十二五"科研面上项目(编号:CWS11J017)
关键词
氯胺酮
抗抑郁作用
前额皮层
谷氨酸
Γ-氨基丁酸
Ketamine
Antidepressant effects
Prefrontal cortex
Glutamate
γ-aminobutyric acid
