摘要
利用光电分析法研究了紫杉醇诱导的非细胞体系中 MCF- 7细胞核的凋亡。在MCF- 7细胞核凋亡过程中 ,光电流的降低与 DNA断裂和细胞核形态学的变化结果相一致。与传统的琼脂糖凝胶电泳法和荧光显微镜观察法相比 ,光电分析法能快速灵敏地在早期检测到细胞核凋亡的动力学信息 ,并适时追踪细胞核凋亡的整个过程。
A new photoelectric method for analyzing cell free apoptosis of MCF 7 nuclei induced by Taxol had been developed in this paper. During the apoptosis of MCF 7 nuclei, the photoelectric current had a dynamic decrease, which was in accordance with the results of fluorescence microscopy and agarose gel electrophoresis. This photoelectric analytical method might provide a rapid and sensitive way to evaluate apoptosis in an early time and in a continuous fashion.
出处
《分析科学学报》
CAS
CSCD
2000年第4期265-269,共5页
Journal of Analytical Science
基金
国家自然科学基金
