摘要
目的:分析甲状腺乳头状癌组织中差异表达小分子RNA(miRNAs)并预测其靶基因,寻找影响甲状腺乳头状癌(PTC)发生发展及可用于生物标志物的miRNAs。方法:选取经病理证实的甲状腺乳头状癌组织及配对正常组织切除标本,运用高通量基因芯片的方法对差异表达的基因和miRNAs进行筛选,采用KEGG通路分析差异表达基因的功能,通过预测网站对差异表达miRNA进行靶基因预测,并对分析结果进行qRT-PCR验证。结果:与同源正常组织比较,基因芯片检测出PTC组织有248个miRNAs(P<0.01)和3 631个基因差异表达(P<0.05)。hsa-miR-101[靶基因:整合素3(ITGA3)]已验证,癌组织中hsa-miR-101表达(59.8%)低于正常甲状腺组织,ITGA3表达(100%)高于正常甲状腺组织,并且与正常组织比较,59.8%PTC组织hsa-miR-101表达下调,同时ITGA3表达上调。结论:hsa-miR-101的靶基因可能为ITGA3,两者在PTC的发生发展中可能发挥重要作用。
Objective To study the differential expression of miRNAs and their target genes in papillary thyroid carcinoma （PTC） tissue, and to analyze the potential roles of miRNAs as biomarkers and in carcinogenesis of PTC. Methods The PTC samples and their matching normal thyroid tissues were examined and collected. The genes and miRNA expression profiles were examined with Illumina Bead Chips. KEGG pathway was used to analyze the function of differential expression genes. MiRNA target genes were predicted by implementing three computational analysis programs, the results were verified by qRT-PCR. Results 248 miRNAs and 3 631 genes were found to he differentially expressed （gene： P〈0.05, miRNA： P〈0.01） in PTC tissues as compared with their matching normal thyroid tissues. Hsa-miR-101 （target gene： ITGA3） was identified. The hsa-miR-101 expression in PTC tissue （59.8 %） was down-regulated and ITGA3s expression （100%） was up-regulated compared with their matching normal thyroid tissues. Oonclusion ITGA3 may be the target gene of hsa-miR-101, and both of them may play important roles in occurrence and development of PTC.
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2013年第1期99-103,共5页
Journal of Jilin University：Medicine Edition
基金
吉林省科技厅科研基金资助课题(201205008)
吉林省卫生厅科技基金资助课题(2010Z005)
吉林大学博士研究生交叉学科基金资助课题(450060483092)
