摘要
采用碱性蛋白酶对鮰鱼皮明胶进行水解,先确定水解度的测定方法,并通过单因素试验和正交试验对碱性蛋白酶水解鮰鱼明胶的工艺条件进行优化。结果显示:利用改进的紫外分光光度法测定鮰鱼皮明胶水解液的水解度,此方法操作简单、结果较准确,可快速测定明胶的水解度。碱性蛋白酶水解明胶的最佳工艺条件为加酶量4000U/mL、酶解温度50℃、pH9.5、酶解时间3h,此时明胶的水解度达到35.12%。
Alkaline protease was used to hydrolyze gelatin from channel catfish skin.The degree of hydrolysis of gelatin was determined by UV spectrophotometry with modifications.The modified method was simple,accurate and rapid.The hydrolysis conditions of alkaline protease were optimized by one-factor-at-a-time method and orthogonal array design.The results showed that the optimum conditions for hydrolyzing gelatin with alkaline protease were obtained as follows：4000 U/mL enzyme loading,50 ℃,pH 9.5 and 3 h.Under the optimized conditions,the degree of hydrolysis of gelatin was 35.12%.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2013年第5期156-160,共5页
Food Science
基金
安徽省自然科学基金项目(090411018)
安徽高校省级自然科学研究重点项目(KJ2009A106)
国家自然科学基金项目(31000832)
关键词
碱性蛋白酶
水解明胶
紫外分光光度法
水解度
alkaline protease
gelatin hydrolysate
UV spectrophotometry
degree of hydrolysis
