摘要
目的进一步了解副溶血弧菌性食物中毒患者粪便与可疑食物中的副溶血弧菌血清型和毒力基因分布。方法细菌分离改用L棒推凃其余参照GB/T4789.7-2003方法,对鉴定为副溶血性弧菌菌株做血清分型和毒力基因(tdh和trh)检测。结果从4份粪便样品和2份从可疑食品中共检出副溶血弧菌28株可分15个血清型,来源于粪便样本的17株副溶血弧菌tdh阳性、trh阴性,来源于可疑食品中的11株副溶血弧菌tdh与trh均阴性。结论该次食物中毒是由一组携带tdh毒力基因多种血清型混合的副溶血弧菌污染引起。
OBJECTIVE To further understand the serotypes and virulence genes in Vibrio parahaemolyticus isolated from feces of an food poisoning patient and suspected food. METHODS Bacteria were isolated through petri dish by bent glass rods, the rest isolation steps followed the GB/T4789. 7 2003. Serotyping and virulence genotyping （tdh and trh） were performed for the identified V. parahaemoZyticus strains. RESULTS From four feces specimens and two suspected food specimens, totally 28 V. parahaemolyticus strains with 15 serotypes were identified. Seventeen strains were from feces specimens, all of them were tdh positive and trh negative. The rest 15 strains from the suspected food specimens were both tdh and trh negative. CONCLUSION The contamination of a group of tdh-positive V. parahaemolyticus with mixed serotypes leads to the food poisoning incidence.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2013年第6期1478-1480,共3页
Chinese Journal of Nosocomiology
基金
浙江省科技厅项目(2008c32035)
关键词
食物中毒
副溶血性弧菌
血清分型
毒力基因检测
Food poisoning
Vibrio parahaemolyticus
Serotyping
Detection of virulence gene
