摘要
本文对我国无锡分离的兔出血症病毒A_2R-3毒株核酸的某些理化性质进行了研究。采用孚尔根染色、二苯胺反应和核酸酶解实验证实病毒核酸为DNA类型。吖啶橙染色、甲醛反应、核酸酶S_1消化和核酸热变性实验表明病毒核酸为单链型。核酸电泳呈单一组分。电境观察显示核酸分子链呈线状,平均长度约为2.15μ。计算分子量约为2.1—2.5×10~6d。核酸碱基组盛为A25.34、T29.37、G23.85、C21.43、(G+C)克分子百分比值为45.28。结合以前的报道、我们认为:兔出血症病毒可以归类于细小病毒科。
In present paper, some biophysical and biochemical characteristies of the nucleic acid in the original strain of the Rabbit Hemerrhagic Disease Virus (RHDV)-A_2R-3(separeted from Wuxi, China. 1984) have been studied.
By using Feulgen assay, Diphenylamine reaction test and nucleate digestion sensitivity test, we demonstrated that the nucleic acid of the RHDV was DNA form.
Staining with acridine orange, reaction with diluted formaldehyde, digestion with nuclease Sl and determination of the thermal denaturation of the RHDV DNA showed that the RHDV contains single-stranded DNA(ssDNA).
Electron micrograph revealed that the RHDV DNA is linear single-strand DNA molecules with 2.15μ in length,their molecular weight is about 2.1—2.5×10~6d.
The base composition is: A 25.34,T 29.37,G 23.38, C 21.43。The G+C mol％ is 45.28.
The results suggested that the RHDV can be classificated into the Parvoviridae and as a member of the Parvoviruses.
基金
国家自然科学基金
关键词
兔出血症病毒
核酸
理化性质
Rabbit Hemorrhagic Disease Virus (RHDV) Parvovirus Single stranded DNA(ssDNA)
