摘要
乙型肝炎病毒C基因3’端切去至少119个碱基,加上终止信号,插入到质粒pBV220中,它具有噬菌体P_RP_L串联启动子和CItS857温度敏感基因,表达条件从30℃上升到 42℃。HBeAg获得高效表达,ELISA效价大于40 000,只含少量HBeAg。用抗-HBe和抗-HBc对表达的HBeAg做阻断抑制试验显示,表达的HBeAg与血清HBeAg特异性完全相同,分子量在15 000道尔顿左右,表达产物呈稳定状态。
Hepatitis D virus C gene was deleted at 3' terminal for at least 119 base pairs, linked to the stop coadon and inserted into plasmid pBV220 which has phage PRPL promoter and CIts 857 temperature sensitive gene. Shifing the culture temperature from 30℃ to 42℃ led to a high level expression of HBeAg.This synthesized material contains only little HBcAg. The pattern of inhibition tests by HBeAb and HBcAb to the expressed HBeAg showed the same characteristics with the serum HBeAg. The molecular weight of the expressed HBeAg is about 15,000 daltons and it is stable on storage at 4℃ for at least 4 months.
出处
《病毒学报》
CAS
CSCD
北大核心
1991年第4期323-330,共8页
Chinese Journal of Virology
关键词
乙肝病毒
E抗原
基因表达
HBV e antigen Gene expression HBV c antigen
