摘要
肠道传播的非甲非乙型肝炎是通过粪口途径传播的。在南亚、中亚细亚和东南亚诸国,东非及我国新疆南部地区都曾发生过大流行,对人民健康危害极大。 该病的病毒(HEV)为直径35nm的廿面体,核酸为正链单链RNA。病毒对理化因子的抵抗力明显不同于肠道病毒,在粪便中极易降解,在常温和-20℃也很快裂解,在组织培养上尚不能繁殖。在一般粪便标本中,HEV的量和RNA含量都较低。因此,用常规的聚合酶链反应(PCR)不能从粪便标本中获得HEV的基因放大物。最近,美国基因实验室(Genelab.)建立了核酸序列非依赖性基因放大法(Sequence independent single
Using the sequence independent single primer gene amplification method a 2,000bp 3' cDNA clone was isolated from the faeces collected from a acutely infected Chinese HEV patient and from the faeces, liver tissue of experimentally infected Rhesus monkey.The specificity of the clone isolated was confirmed by probes Hp1, Hp2, pF2 and pR3, which represented the more conservative sequences in p part of the HEV genome cloned from the faeces of a Burmese HEV patient.It was demonstrated that the SISpA products could be hybridized with all four probes,except that the pR3 probe gave a weaker reaction with the SISpA products.
出处
《病毒学报》
CAS
CSCD
北大核心
1991年第1期66-68,共3页
Chinese Journal of Virology
关键词
非甲非乙肝炎
基因克隆
HEV
Hepatitis E Virus Sequence independent single primergene amplification method
