摘要
以早钟6号枇杷的试管苗叶片为材料,采用同源克隆方法得到乙烯受体基因ETR的两个成员Ej-ETR1a和Ej-ETR2a的cDNA全长序列,在GenBank上的登录号为JX307084和JX307089。Ej-ETR1a序列全长2 771 bp,包括227 bp 5′-UTR、2 226 bp ORF及318 bp 3′-UTR;Ej-ETR2a序列全长2 594 bp,包含2 226 bp ORF及366 bp 3′-UTR。Ej-ETR1a、Ej-ETR2a均编码741个氨基酸,且氨基酸序列具有95.28%的相似性。生物学信息分析表明:枇杷Ej-ETR1a和Ej-ETR2a均属于乙烯受体ETR1亚家族,为一个具有跨膜结构的非分泌蛋白,具有疏水性。2个ETR成员的克隆为进一步研究枇杷乙烯受体作用机制奠定了基础。
Two ethylene receptor genes, Ej-ETRla and Ej-ETR2a （assession No. JX307084 and JX307089 in GneBank, respectively）, were cloned by homologous cloning from the leaves of the in vitro plantlets in Eriobotrya japonica cv. Zaozhong No.6. The length of Ej-ETRla was 2 771 bp, including a 5＇ untranslated region of 227 bp, an open reading frame of 2226bp and a 3＇ untranslated region of 318 bp; the length of Ej-ETR2a was 2 594bp, and it was consisted of an open reading frame of 2 226 bp and a 3＇ untranslated region of 366 bp. Both Ej-ETRla and Ej-ETR2a encoded 741 amino acids, and the homology of their translation of amino acids was 95.28%. Bioinformatic analysis showed that the Ej-ETRla and Ej-ETR2a belonged to the ETR1 subgroups, and they were hydrophobic and without the signal peptide, and belonged to the transmembrane protein. The cloning of two ethylene receptor genes provided a foundation for further study of functional mechanism of the ethylene receptors in E. japonica.
出处
《热带作物学报》
CSCD
北大核心
2013年第2期276-284,共9页
Chinese Journal of Tropical Crops
基金
福建省重大科技平台建设(No.2008N2001)项目
国家科技支撑计划(No.2007BAD07B01)项目
关键词
枇杷
ETR
基因克隆
序列分析
Eriobotrya japonica
Ethylene receptor
Gene cloning
Sequence analysis
